Tertiary motifs in RNA structure and folding

RT Batey, RP Rambo… - Angewandte Chemie …, 1999 - Wiley Online Library
… The GNRA sequence motif, in which any nucleotide is accommodated in the second position
and the third position is a purine base (R ŕ A or G), is the most prevalent tetraloop motif in …

Super-resolution in solution X-ray scattering and its applications to structural systems biology

RP Rambo, JA Tainer - Annual review of biophysics, 2013 - annualreviews.org
… Similarly, a comparison of the protein's R g to Flory's … , to R g RC for a random coil) can be
used to infer flexibility (12, 78). An R g < R g RC suggests compactness and likewise R g > R g …

Bridging the solution divide: comprehensive structural analyses of dynamic RNA, DNA, and protein assemblies by small-angle X-ray scattering

RP Rambo, JA Tainer - Current opinion in structural biology, 2010 - Elsevier
… estimation of the radius-of-gyration (R g ) within the Guinier region of the data (q·R g … R g
describes the mass distribution of a macromolecule about its center of gravity. An increase in R

Accurate assessment of mass, models and resolution by small-angle scattering

RP Rambo, JA Tainer - Nature, 2013 - nature.com
… We discovered that a parameter, Q R , defined as the ratio of the square of V c to R g with
units of Ĺ 3 , is linear versus molecular mass in a log–log plot (Figs 2, 3 and Supplementary Fig…

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

GL Hura, AL Menon, M Hammel, RP Rambo… - Nature …, 2009 - nature.com
… with R G > 75 Ĺ and a longest dimension across the molecule (D max ) of at least 340 Ĺ (larger
than a ribosome). Scattering curve oscillations beyond q > 0.1 Ĺ −1 with such a large R G …

Structural mechanism of abscisic acid binding and signaling by dimeric PYR1

N Nishimura, K Hitomi, AS Arvai, RP Rambo, C Hitomi… - Science, 2009 - science.org
The phytohormone abscisic acid (ABA) acts in seed dormancy, plant development, drought
tolerance, and adaptive responses to environmental stresses. Structural mechanisms …

Characterizing flexible and intrinsically unstructured biological macromolecules by SAS using the Porod‐Debye law

RP Rambo, JA Tainer - Biopolymers, 2011 - Wiley Online Library
… This method normalizes the scattering data to I(0) and skews the scattering angle by R g (Figure
3C), such that the resulting SAS data in a Kratky plot is normalized for c, V, and R g …

Crystal structure of the ribonucleoprotein core of the signal recognition particle

RT Batey, RP Rambo, L Lucast, B Rha, JA Doudna - Science, 2000 - science.org
… Using diffraction data extending from 30.0 to 1.8 Å resolution, a model of the complex was
built and refined to a crystallographic R factor of 19.9% and a freeR factor of 22.1% (Table 1). …

Structural basis of Smoothened regulation by its extracellular domains

…, RP Rambo, MSP Sansom, S Newstead, R Rohatgi… - Nature, 2016 - nature.com
… either CRD–linker domain contacts or the linker domain itself increases the constitutive
activity of SMO, implicating these regions in stabilizing an inactive state, analogous to the D(E)R

SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication

R Bétous, AC Mason, RP Rambo… - Genes & …, 2012 - genesdev.cshlp.org
… The radius of gyration (r g ) obtained from the Guinier region was 33.0 ± 0.3 Ĺ (…
compared with glucose isomerase, a spherical protein at 173 kDa with a similar R g of 32 Ĺ. …