Understanding, improving and using green fluorescent proteins

AB Cubitt, R Heim, SR Adams, AE Boyd… - Trends in biochemical …, 1995 - cell.com
Green fluorescent proteins (GFPs) are presently attracting tremendous interest as the first
general method to create strong visible fluorescence by purely molecular biological means. So …

Understanding structure—function relationships in the Aequorea victoria green fluorescent protein

AB Cubitt, LA Woollenweber, R Heim - Methods in cell biology, 1998 - Elsevier
Publisher Summary Learning the physiological role of green fluorescent protein (GFP) and
its interaction with aequorin could help understand how to create mutants that exhibit …

Crystal Structure of the Aequorea victoria Green Fluorescent Protein

M Ormö, AB Cubitt, K Kallio, LA Gross, RY Tsien… - Science, 1996 - science.org
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria
has generated intense interest as a marker for gene expression and localization of gene …

[HTML][HTML] On/off blinking and switching behaviour of single molecules of green fluorescent protein

RM Dickson, AB Cubitt, RY Tsien, WE Moerner - Nature, 1997 - nature.com
Optical studies of individual molecules at low and room temperature can provide information
about the dynamics of local environments in solids, liquids and biological systems …

Inhibition of VEGF binding to neuropilin-2 enhances chemosensitivity and inhibits metastasis in triple-negative breast cancer

…, S Klopp-Savino, S Paz, M Zhang, A Cubitt… - Science translational …, 2023 - science.org
Although blocking the binding of vascular endothelial growth factor (VEGF) to neuropilin-2 (NRP2)
on tumor cells is a potential strategy to treat aggressive carcinomas, a lack of effective …

[HTML][HTML] Serum-circulating His-tRNA synthetase inhibits organ-targeted immune responses

…, J Ampudia, S Rosengren, A Cubitt… - Cellular & molecular …, 2021 - nature.com
His-tRNA synthetase (HARS) is targeted by autoantibodies in chronic and acute inflammatory
anti-Jo-1-positive antisynthetase syndrome. The extensive activation and migration of …

Apparent adiposity assessed by standardised scoring systems and morphometric measurements in horses and ponies

RA Carter, RJ Geor, WB Staniar, TA Cubitt… - The Veterinary Journal, 2009 - Elsevier
This study described a scoring system for the assessment of apparent neck adiposity and
evaluated morphometric measurements for assessment of neck and overall adiposity. Twenty-…

Developing correctional policy, practice, and clinical care considerations for incarcerated transgender patients through collaborative stakeholder engagement

NE Kendig, A Cubitt, A Moss… - Journal of Correctional …, 2019 - liebertpub.com
Transgender persons are at increased risk of victimization during incarceration and have
unique health care needs. We convened a symposium of 27 key stakeholders to develop …

Patterns of free calcium in multicellular stages of Dictyostelium expressing jellyfish apoaequorin

AB Cubitt, RA Firtel, G Fischer, LF Jaffe… - …, 1995 - journals.biologists.com
To examine the patterns of high free cytosolic calcium or [Ca 2+ ] i during Dictyostelium’s
development, we expressed apoaequorin in D. discoideum, reconstituted aequorin and …

Amino acid substitutions in the Dictyostelium G alpha subunit G alpha 2 produce dominant negative phenotypes and inhibit the activation of adenylyl cyclase, guanylyl …

K Okaichi, AB Cubitt, GS Pitt… - Molecular biology of the …, 1992 - Am Soc Cell Biol
Previous studies have demonstrated that the Dictyostelium G alpha subunit G alpha 2 is
essential for the cAMP-activation of adenylyl cyclase and guanylyl cyclase and that g alpha 2 …