Respiratory input impedance (Zin) over a wide range of frequencies (f) has been shown to be useful in determining airway resistance (Raw) and tissue resistance in dogs or airway wall properties in human adults. Zin measurements are noninvasive and, therefore, potentially useful in investigation of airway mechanics in infants. However, accurate measurements of Zin at these f values with the use of forced oscillatory techniques (FOT) in infants are difficult because of their relatively high Raw and large compliance of the face mask. If pseudorandom noise pressure oscillations generated by a loudspeaker are applied at the airway opening (FOT), the power of the resulting flow decreases inversely with f because of capacitive shunting into the volume of the gas in the speaker chamber and in the face mask. We studied whether high-frequency respiratory Zin can be measured by using rapid flow interruption [high-speed interrupter technique (HIT)], in which we expect the flow amplitude in the respiratory system to be higher than in the FOT. We compared Zin measured by HIT with Zin measured by FOT in a dried dog lung and in five healthy adult subjects. The impedance was calculated from two pressure signals measured between the mouth and the HIT valve. The impedance could be assessed from 32 to 800 Hz. Its real part at low f as well as the f and amplitude of the first and second acoustic resonance, measured by FOT and by HIT, were not significantly different. The power spectrum of oscillatory flow when the HIT was used showed amplitudes that were at least 100 times greater than those when FOT was used, increasing at f > 400 Hz. In conclusion, the HIT enables the measurement of high-frequency Zin data ranging from 32 to 800 Hz with particularly high flow amplitudes and, therefore, possibly better signal-to-noise ratio. This is particularly important in systems with high Raw, e.g., in infants, when measurements have to be performed through a face mask.