Abstract
The functional necessity for two CD28 counterreceptors (B7-1 and B7-2) is presently unknown. B7-1 and B7-2 equivalently costimulate IL-2 and interferon-gamma (IFN gamma) production and IL-2 receptor alpha and gamma chain expression. B7-2 induces significantly more IL-4 production than B7-1, with the greatest difference seen in naive T cells. Repetitive costimulation of CD4+ CD45RA+ T cells with B7-2 results in moderate levels of both IL-4 and IL-2, whereas repetitive costimulation with B7-1 results in high levels of IL-2 and low levels of IL-4. Therefore, B7-1 and B7-2 costimulation mediate distinct outcomes, since B7-2 provides an initial signal to induce naive T cells to become IL-4 producers, thereby directing the immune response more towards Th0/Th2, whereas B7-1 is a more neutral differentiative signal.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Antigens, CD*
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B7-1 Antigen / physiology*
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B7-2 Antigen
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CD4-Positive T-Lymphocytes / chemistry
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CD4-Positive T-Lymphocytes / physiology*
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CHO Cells
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Cell Differentiation / drug effects
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Cells, Cultured
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Cricetinae
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Gene Expression
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Humans
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Immunophenotyping
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In Vitro Techniques
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Interferon-gamma / biosynthesis
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Interleukin-2 / biosynthesis
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Interleukin-4 / biosynthesis*
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Leukocyte Common Antigens / metabolism
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Lymphocyte Culture Test, Mixed
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Lymphotoxin-alpha / biosynthesis
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Membrane Glycoproteins / physiology*
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Mice
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RNA, Messenger / genetics
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Receptors, Interleukin-2 / metabolism
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T-Lymphocyte Subsets / metabolism
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Transfection
Substances
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Antigens, CD
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B7-1 Antigen
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B7-2 Antigen
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CD86 protein, human
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Cd86 protein, mouse
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Interleukin-2
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Lymphotoxin-alpha
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Membrane Glycoproteins
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RNA, Messenger
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Receptors, Interleukin-2
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Interleukin-4
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Interferon-gamma
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Leukocyte Common Antigens