Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores

Nature. 1989 May 4;339(6219):64-7. doi: 10.1038/339064a0.

Abstract

The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens / immunology
  • Arachidonic Acid
  • Arachidonic Acids / metabolism
  • Calcimycin / pharmacology
  • Calcium / metabolism
  • Cell Line
  • Cross-Linking Reagents
  • Cyclosporins / pharmacology
  • Cytosol / metabolism
  • DNA / biosynthesis
  • Dinitrophenols / immunology
  • Dinitrophenols / pharmacology
  • Ethers / pharmacology
  • Histamine Release
  • Immunoglobulin E / immunology
  • Interleukin-3 / biosynthesis
  • Interleukin-4
  • Interleukin-5
  • Interleukin-6
  • Interleukins / biosynthesis
  • Ionomycin
  • Lymphokines / biosynthesis*
  • Lymphokines / genetics
  • Mast Cells / drug effects
  • Mast Cells / immunology
  • Mast Cells / metabolism*
  • Mice
  • RNA, Messenger / biosynthesis
  • Receptors, Fc / metabolism*
  • Serum Albumin, Bovine / immunology
  • Serum Albumin, Bovine / pharmacology

Substances

  • Antigens
  • Arachidonic Acids
  • Cross-Linking Reagents
  • Cyclosporins
  • Dinitrophenols
  • Ethers
  • Interleukin-3
  • Interleukin-5
  • Interleukin-6
  • Interleukins
  • Lymphokines
  • RNA, Messenger
  • Receptors, Fc
  • dinitrophenyl-bovine serum albumin
  • Interleukin-4
  • Serum Albumin, Bovine
  • Arachidonic Acid
  • Immunoglobulin E
  • Calcimycin
  • Ionomycin
  • DNA
  • Calcium