Abstract
The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens / immunology
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Arachidonic Acid
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Arachidonic Acids / metabolism
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Calcimycin / pharmacology
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Calcium / metabolism
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Cell Line
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Cross-Linking Reagents
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Cyclosporins / pharmacology
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Cytosol / metabolism
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DNA / biosynthesis
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Dinitrophenols / immunology
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Dinitrophenols / pharmacology
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Ethers / pharmacology
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Histamine Release
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Immunoglobulin E / immunology
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Interleukin-3 / biosynthesis
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Interleukin-4
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Interleukin-5
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Interleukin-6
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Interleukins / biosynthesis
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Ionomycin
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Lymphokines / biosynthesis*
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Lymphokines / genetics
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Mast Cells / drug effects
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Mast Cells / immunology
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Mast Cells / metabolism*
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Mice
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RNA, Messenger / biosynthesis
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Receptors, Fc / metabolism*
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Serum Albumin, Bovine / immunology
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Serum Albumin, Bovine / pharmacology
Substances
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Antigens
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Arachidonic Acids
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Cross-Linking Reagents
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Cyclosporins
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Dinitrophenols
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Ethers
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Interleukin-3
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Interleukin-5
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Interleukin-6
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Interleukins
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Lymphokines
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RNA, Messenger
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Receptors, Fc
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dinitrophenyl-bovine serum albumin
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Interleukin-4
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Serum Albumin, Bovine
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Arachidonic Acid
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Immunoglobulin E
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Calcimycin
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Ionomycin
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DNA
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Calcium