Abstract
Several matrix metalloproteinase (MMP) members contribute to pathology in multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). As the role of MMP-12 in EAE has been understudied, we examined its expression in EAE and in vitro. MMP-12 transcripts increased with EAE, and protein was localized to a subset of macrophages/microglia. The temporal expression of MMP-12 largely corresponded to that of cytokines, and we demonstrate that IL-1beta and TNF-alpha promoted MMP-12 expression in cultured macrophages. We postulate that cytokine - MMP-12 interactions are important in the disease process of EAE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Encephalomyelitis, Autoimmune, Experimental / enzymology*
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Encephalomyelitis, Autoimmune, Experimental / immunology*
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Encephalomyelitis, Autoimmune, Experimental / pathology
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Female
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Fluorescent Antibody Technique
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Gene Expression / immunology
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In Vitro Techniques
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Interferon-gamma / biosynthesis
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Interleukin-1beta / biosynthesis
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Macrophages / enzymology*
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Macrophages / immunology*
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Matrix Metalloproteinase 12 / biosynthesis*
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Mice
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Mice, Inbred C57BL
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Reverse Transcriptase Polymerase Chain Reaction
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Spinal Cord / immunology
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Spinal Cord / metabolism
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Spinal Cord / pathology
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Tumor Necrosis Factor-alpha / biosynthesis
Substances
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Interleukin-1beta
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Tumor Necrosis Factor-alpha
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Interferon-gamma
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Matrix Metalloproteinase 12