Abstract
To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chlorocebus aethiops
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Culture Media
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DNA, Viral / genetics
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DNA, Viral / isolation & purification
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Herpesvirus 1, Human / genetics
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Herpesvirus 1, Human / isolation & purification
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Humans
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Microbiological Techniques / methods*
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Microbiological Techniques / statistics & numerical data
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Nucleic Acid Amplification Techniques / methods*
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Nucleic Acid Amplification Techniques / statistics & numerical data
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Polymerase Chain Reaction
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Sensitivity and Specificity
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Vero Cells