Abstract
The performance characteristics of the enzyme-linked immunospot assay (ELISPOT) assay (T-SPOT TB) for the diagnosis of latent tuberculosis infection in HIV-infected individuals are unknown. Given that ELISPOT enumerates Mycobacterium tuberculosis antigen-specific IFN-gamma-secreting T cells, HIV-associated immunosuppression might adversely affect test performance. However, we found that 28 out of 29 HIV-positive individuals (97%) gave evaluable test results, and performance was independent of the CD4 T-cell count. ELISPOT test performance appears to be independent of HIV-associated immunosuppression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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CD3 Complex / immunology
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CD4 Lymphocyte Count
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Diagnostic Tests, Routine / methods
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Enzyme-Linked Immunosorbent Assay / methods
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Female
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HIV Infections / complications*
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HIV Infections / immunology
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Humans
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Immune Tolerance / immunology
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Interferon-gamma / immunology
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Interleukin-2 / immunology
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Interleukin-4 / immunology
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Lymphocyte Count
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Male
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Phytohemagglutinins / immunology
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RNA, Messenger / blood
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RNA, Viral / blood
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T-Lymphocytes / immunology*
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Tuberculosis / diagnosis*
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Tuberculosis / immunology
Substances
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CD3 Complex
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Interleukin-2
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Phytohemagglutinins
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RNA, Messenger
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RNA, Viral
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Interleukin-4
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Interferon-gamma