The accurate quantification of right ventricular (RV) mass has eluded conventional imaging modalities. Accordingly, cine magnetic resonance imaging was used for quantification of RV as well as left ventricular (LV) mass in 10 normal subjects and in 10 patients with dilated cardiomyopathy with an LV ejection fraction less than 0.40. Hearts were imaged with 10 mm thick short-axis slices from apex to base with a short echo delay time of 5 ms. Each slice was partitioned into 3 sections: RV free wall, ventricular septum and LV free wall, for calculation of end-diastolic and end-systolic mass and LV:RV free wall ratio. RV end-diastolic mass in normal subjects was 45 +/- 8 g, which was similar to the values determined in previously published postmortem studies, mean 46 g (range 23 to 68). The value determined in patients with dilated cardiomyopathy was higher (50 +/- 11 g), but this difference was not significant. LV:RV free wall ratio in cardiomyopathy (3.6 +/- 1.0) was greater than in normal subjects (2.4 +/- 0.3), because of the greater LV free wall mass in dilated cardiomyopathy, where LV free wall end-diastolic mass was 173 +/- 40 g vs 107.1 +/- 19.9 g in normal subjects (p less than 0.05). RV mass measurements had 6.4 +/- 3.6% interobserver and 7.3 +/- 6.1% intraobserver variability. There were no significant differences between end-diastolic and end-systolic mass measurements. Thus, cine magnetic resonance imaging can reproducibly calculate RV mass. The values in normal subjects correspond to previously reported postmortem values for a population without heart disease.