Abstract
Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family proteins, which have diverse roles in regulation of gene expression and cellular functions. We found RIG-I mRNA and protein were expressed in MCF-7 human breast cancer cells stimulated with interferon-gamma (IFN-gamma). This effect of IFN-gamma was observed in concentration- and time-dependent manners, and IFN-gamma also induced promoter activity of RIG-I. Transfection of GFP-RIG-I cDNA into MCF-7 cells resulted in the expression of RIG-I protein in cytoplasm. Overexpression of RIG-I induced the upregulation of IFN-gamma stimulated gene 15, which has the potential to amplify the immunomodulatory effects. We conclude that IFN-gamma induces the expression of RIG-I, which may play a role in the immunological effects of IFN-gamma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiviral Agents / pharmacology*
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Breast Neoplasms / metabolism*
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Breast Neoplasms / pathology
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Cell Line, Tumor
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Cytokines / biosynthesis*
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Cytokines / genetics
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Cytoplasm / metabolism
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DEAD Box Protein 58
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DEAD-box RNA Helicases
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Female
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / genetics
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Humans
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Interferon-gamma / pharmacology*
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Promoter Regions, Genetic / genetics*
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RNA Helicases / biosynthesis*
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RNA Helicases / genetics
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RNA, Messenger / biosynthesis
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Receptors, Immunologic
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Ubiquitins / analogs & derivatives*
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Ubiquitins / biosynthesis*
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Ubiquitins / genetics
Substances
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Antiviral Agents
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Cytokines
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RNA, Messenger
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Receptors, Immunologic
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Ubiquitins
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ISG15 protein, human
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Interferon-gamma
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RIGI protein, human
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DEAD Box Protein 58
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DEAD-box RNA Helicases
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RNA Helicases