Hydrogen peroxide sensitive amperometric biosensor based on horseradish peroxidase entrapped in a polypyrrole electrode

S Serradilla Razola; B Lopez Ruiz; N Mora Diez; H B Mark Jr; J M Kauffmann

doi:10.1016/s0956-5663(02)00083-0

Hydrogen peroxide sensitive amperometric biosensor based on horseradish peroxidase entrapped in a polypyrrole electrode

Biosens Bioelectron. 2002 Dec;17(11-12):921-8. doi: 10.1016/s0956-5663(02)00083-0.

Authors

S Serradilla Razola¹, B Lopez Ruiz, N Mora Diez, H B Mark Jr, J M Kauffmann

Affiliation

¹ Institut de Pharmacie, Université Libre de Bruxelles, Campus Plaine, CP 205/6, 1050 Brussels, Belgium.

PMID: 12392940
DOI: 10.1016/s0956-5663(02)00083-0

Abstract

The enzyme horseradish peroxidase (HRP) has been entrapped in situ by electropolymerization of pyrrole onto a platinum electrode. The latter was previously coated by a polypyrrole layer for better adhesion of the biocatalyst film and in order to avoid the enzyme folding onto the Pt electrode. The biosensor allowed the determination of hydrogen peroxide in the concentration range comprised between 4.9 x 10(-7) and 6.3 x 10(-4) M. The biosensor retained more than 90% of its original activity after 35 days of use.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Coated Materials, Biocompatible
Electrochemistry / instrumentation*
Electrochemistry / methods
Electrodes*
Enzyme Stability
Enzymes, Immobilized / chemistry
Equipment Failure Analysis
Horseradish Peroxidase / chemistry*
Hydrogen Peroxide / analysis*
Hydrogen Peroxide / chemistry
Hydrogen-Ion Concentration
Platinum
Polymers*
Pyrroles*
Reproducibility of Results
Sensitivity and Specificity

Substances

Coated Materials, Biocompatible
Enzymes, Immobilized
Polymers
Pyrroles
polypyrrole
Platinum
Hydrogen Peroxide
Horseradish Peroxidase