Chest
Laboratory and Animal InvestigationsStatic Inflation Attenuates Ischemia/Reperfusion Injury in an Isolated Rat Lung In Situ
Section snippets
Preparation of the Isolated Lung
The preparation of an isolated rat lung in situ model has been described previously.9161718 In brief, a total of 96 male Sprague-Dawley rats weighing 250 to 350 g were anesthetized intraperitoneally with sodium pentobarbital (30 mg/kg) and were intubated with an endotracheal tube. A rodent ventilator provided ventilation with a mixture of 95% room air and 5% carbon dioxide. The respiratory rate was set at 60 to 65 breaths/min, and tidal volume was set at 2 to 3 mL. The inspiratory pressure and
Kfc
Figure 1illustrates Kfc changes by I/R in the isolated lung perfused with whole blood or KHS. I/R caused a marked increase in Kfc. Static lung inflation significantly attenuated the increase in Kfc. The mean Kfc change in the lung perfused with whole blood (1.54 ± 0.46 g/min/cm H2O per 100 g) was lower than that in the lung perfused with KHS (2.31 ± 0.18 g/min/cm H2O per 100 g; p < 0.05).
LWG and LW/BW Ratio
Figure 2shows that I/R increased LWG during the R period for 50 min. Static inflation significantly reduced
Discussion
Previous studies have reported an association between the state of lung inflation and I/R injury. Puskas and coworkers10 noted that donor lung storage in the inflated state during organ perfusion improved the outcome. Stevens et al11 also reported improved lung preservation by prevention of lung collapse. However, there have also been conflicting reports1213 indicating that hyperinflation of the preserved lung led to a worse outcome. In fact, Aoe et al14 showed that immediate postpreservation
ACKNOWLEDGMENT
The authors are grateful to Liu Yen Chin for his excellent assistance in the technique used, to Lucy Chen from Simon Fraser University (Burnaby, BC, Canada) for improving the English writing, to Dr. Y. W. Lee for her help in the statistical analysis, and to C. C. Chang for the preparation of this manuscript.
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This study was supported in part by grants from the National Science Council (90–2320-B-320–002, 90–2316-B-320–004, and NSC 91–2320-B-320–008), and by the Shin Kong Wu-Ho Su Memorial Foundation and the Outstanding Scholarship Development Foundation (1996–2001).