Chest
Clinical InvestigationsTuberculosisEvaluation of Polymerase Chain Reaction for Detection of Mycobacterium tuberculosis in Pleural Fluid
Section snippets
Patients and Pleural Fluid
The population under study comprised 60 patients with pleural effusion. The study period was from July 1997 to December 1998. Any patient coming to the outpatient department within the study period with pleural effusion and with no other specific clinical history for disease, such as lung cancer, and who had not received any antituberculous treatment (ATT) was included in the study. Pleural fluid was aspirated from these patients and transported to the laboratory for investigation.
Study Design
The study was
Results
Sixty samples of pleural effusion were obtained for this study from the outpatient unit of the Department of Pulmonary Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India. Twenty samples were tubercular pleural effusion, and 40 samples were nontubercular pleural effusion. The latter were obtained as a disease control group. The ages of the patients with tubercular pleurisy (study group) ranged from 6 to 70 years and included 9 female (45%) and 11 male patients
Discussion
Traditional methods of diagnosing tuberculosis have been isolation of bacilli in culture or recognition of AFB in clinical specimens. AFB staining lacks sensitivity. So far, the “gold standard” has been culture. But with a dividing time of 48 h, it takes up to 10 weeks using the culture method. Besides, microscopy-positive specimens fail to yield mycobacterium on culture, perhaps due to harsh chemical treatment used in decontamination or the presence of nonviable mycobacterium in partially
References (22)
- et al.
The detection of mycobacterial DNA sequences in uncultured clinical specimens with cloned Mycobacterium tuberculosisDNA probes
Tubercle
(1988) - et al.
Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation
Lancet
(1991) Adenosine deaminase
Value of direct amplified test for diagnosis of tuberculosis
Lancet
(1996)- et al.
Tuberculous pleurisy
Chest
(1973) - et al.
Enumeration of tubercle bacilli in sputum of patients with pulmonary tuberculosis
Antimicrob Agents Chemother
(1973) New approaches to the rapid diagnosis of tuberculous meningitis
J Infect Dis
(1987)- et al.
Diagnosis of pulmonary tuberculosis by detection of tuberculostearic acid in sputum by using gas chromatography-mass spectrometry with selected ion monitoring
J Infect Dis
(1987) - et al.
Repetitive DNA sequences as probes for Mycobacterium tuberculosis
J Clin Microbiol
(1988) - et al.
A cloned DNA fragment for identification of Mycobacterium tuberculosis
Rev Infect Dis
(1989)
Use of polymerase chain reaction for rapid diagnosis of tuberculosis
J Clin Microbiol
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