Chest
Clinical InvestigationsExpression of Alveolar Macrophage Adhesion Molecules in Pulmonary Sarcoidosis
Section snippets
Study Population
Twenty-three patients with pulmonary sarcoidosis (11 women and 12 men; mean age, 41.0 yr; range, 22 to 62 yr) were investigated. The diagnosis was based on consistent clinical features, along with biopsy evidence of noncaseating epithelioid cell granulomas in 16 cases and the characteristic highly specific increase (greater than 5.0) of the CD4/CD8 ratio in BAL in the remaining seven cases.15 According to radiologic staging, nine were type 1, 14 were type 2 and none was type 3. Twenty patients
Results
The total and differential cell counts and the CD4/ CD8 ratio in BAL fluid of patients with sarcoidosis and control subjects are shown in Table 2. The results of AM phenotyping are shown in Figures 1 and 2.
The percentage of CD11a-positive AM did not significantly differ among patients with active sarcoidosis (94.7 ± 5.1), inactive disease (92.6 ± 7.8) and the control group (89.3 ± 8.7 [ANOVA]).
The expression of CD11b was significantly increased in patients with active disease (83.4 ± 12.1)
Discussion
To our knowledge, this is the first study that analyzed the expression of the complete family of beta-2-integrins and its ligand ICAM-1 on AM in patients with pulmonary sarcoidosis and control subjects. Since corticosteroids have not been shown to modulate the expression of integrins in a previous study,18 patients undergoing treatment with corticosteroids were not excluded from this study. Similarly, we did not exclude smokers, since adherence19 and surface marker expression20 have been
ACKNOWLEDGMENT
The authors thank G. Knautz for technical assistance and J. Reissigova for assistance in the statistical evaluation of the data.
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2009, Clinical ImmunologyCitation Excerpt :Recently, integrins have been an area of interest in (chronic) inflammatory diseases such as multiple sclerosis [16,17] and SLE [18,19]. In sarcoidosis, increased expression of integrins VLA-4 and VLA-5 on BALF lymphocytes has been reported by Berlin et al. [31], and increased expression of β1 and β2-integrins on peripheral blood monocytes and alveolar macrophages has been found in patients with active sarcoidosis compared to patients with inactive disease [32–34]. Interestingly, radiographic staging showed that parenchymal involvement (stages II and higher) correlated with a significantly higher percentage of CD103 positive CD4+ lymphocytes [35,36].
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The study was supported by the AFPR and the DAAD. Supported by grant No. 0071-2 from the Ministry of Health of Czech Republic.