ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
STUDIES ON TRANSLOCATION OF IMMUNOGLOBULINS ACROSS INTESTINAL EPITHELIUM
I. IMPROVEMENTS IN THE PEROXIDASE-LABELED ANTIBODY METHOD FOR APPLICATION TO STUDY OF HUMAN INTESTINAL MUCOSA
YOSHINARI ISOBESHIN-TSE CHENPAUL K. NAKANEWILLIAM R. BROWN
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1977 Volume 10 Issue 2 Pages 161-171

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Abstract

We adapted the peroxidase-labeled antibody method to localization of immunoglobulins and the secretory component (SC) in human intestinal mucosa at the light and electron microscopic levels. Modifications of the method included (1) the use of digitonin in the first wash step of tissues fixed in periodate-lysine-paraformaldehyde; (2) double coating of glass slides with gelatin and egg albumin before application of cyostat sections, and (3) inactivation of endogenous peroxidase by periodic acid treatment of cyostat sections. With these improvements, tissue morphology was well preserved; antigenicity of immunoglobulins and SC was retained; penetration of labeled antibodies into cellular compartments was complete; and confusion at the light microscopic level between peroxidase-containing cells and cells stained with peroxidase-labeled antibodies was avoided.

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© the Japan Society of Histochemistry and Cytochemistry
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