Environmental and Occupational DisordersGlutathione S-transferase GSTP1 is a susceptibility gene for occupational asthma induced by isocyanates☆,☆☆,★
Section snippets
Subject recruitment
The association between GST genotypes and susceptibility was studied in 131 unrelated white European subjects residing in the north of Italy. All were exposed to TDI in furniture or body shops. The 92 asthmatic patients were recruited in the Department of Environmental Medicine and Public Health of the University of Padua, which was established to diagnose occupational asthma induced by exposure to isocyanates. The 39 asymptomatic workers were employed in the same factories for at least 10
GSTP1 genotype frequencies
Table II shows GSTP1 genotype frequencies in the study population. Frequencies of the GSTP1*A, GSTP1*B, GSTP1*C, and GSTP1*D alleles were 0.694, 0.177, 0.069, and 0.044, respectively. GSTP1 genotype frequencies achieved Hardy-Weinberg equilibrium and were similar to those observed in a population of atopic asthmatic subjects, atopic nonasthmatic subjects, and nonatopic asthmatic subjects from North Staffordshire, England, except that the GSTP1*D frequency (0.005) was lower.14
Association ofGSTP1 genotypes with asthma and AHR
GSTP1 genotype
Discussion
We have shown that polymorphism in GSTP1 is associated with susceptibility to asthma induced by prolonged exposure to TDI, a commonly used occupational respiratory sensitizer. We have also demonstrated that homozygosity for the Val 105-encoding alleles confer protection against the development of the asthma-associated phenotype AHR; this protective effect increased in proportion to the duration of TDI exposure. Our data suggest that individuals lacking this genotype may, over time, exhibit
Acknowledgements
We thank Stefano Ferrarese, MD, for his valuable contribution.
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Supported by MURST (60% and 40%), Ministero del Lavoro e della Previdenza Sociale, ARCA, and the British Lung Foundation.
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Received for publication ···;
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Reprint requests: Cristina E. Mapp, MD, Dipartimento di Medicina Clinica e Sperimentale, Sezione di Igiene e Medicina del Lavoro, Via Fossato di Mortara 64/b, 44100 Ferrara, Italy.