Asthma, Rhinitis, Other Respiratory DiseasesArray-based diagnostic gene-expression score for atopy and asthma☆
Section snippets
Subjects
Healthy control subjects, atopic nonasthmatic subjects, and atopic asthmatic subjects were recruited. Healthy control subjects were defined as individuals with negative skin prick test results without medication and without lung or systemic disease. Atopy was defined as a positive skin prick test response (wheal of ≥3 mm diameter larger than that produced by the negative control wheal) to a range of 4 common aeroallergens: Dermatophagoides pteronyssinus , cat, dog, and mixed grasses (ALK).
Results
Fourteen nonatopic healthy control subjects (male/ female ratio, 2:12; mean age, 42 ± 13 years; age range, 27-65 years), 8 atopic subjects without asthma (male/female ratio, 7:1; mean age, 36 ± 9 years; age range, 23-49 years), and 18 atopic asthmatic subjects (male/female ratio, 6:12; mean age, 41 ± 14 years; age range, 18-66 years) were included in the study. Sixteen of 18 asthmatic subjects had inhaled corticosteroids and Aas asthma severity scores ranging from 1 to 5 (score 1, n = 2; score
Discussion
In this study we quantified the overall atopy-specific gene-expression activity with the CAGE score. The CAGE score had a sensitivity of 96% and a specificity of 92% for the diagnosis of atopy and thus was superior to total IgE values. The CAGE score correlated with total IgE levels and a trend for asthma severity was found. Thus the CAGE score provided an overall index of current atopic disease activity.
Gene-expression scores could be used as a clear-cut diagnostic tool for the diagnosis and
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2008, VaccineCitation Excerpt :Immunisation with pertussis vaccines has been reported to induce both a Th1 and Th2 immune response [3]. Recent studies using microarray technology have lead to the discovery of several important markers for asthma and allergy [4–10], but it is unknown if they are expressed during or after immune responses. Pharmacogenomics is ideally suited to study cellular responses related to immune response and it was shown earlier that cytokine markers measured on the gene expression level by array technology are in good correlation with the corresponding protein levels measured by conventional technology [11].
Gene expression profiling as novel tool in experimental asthma research
2006, Experimental and Toxicologic Pathology
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Reprint requests: Martin H. Brutsche, MD, PhD, Pneumology, University Hospital, Petersgraben 4, CH-4031 Basel, Switzerland.