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Cytokines

TNFα, interleukin-10 and interleukin-18 expression in cells of the bronchoalveolar lavage in patients with pulmonary complications following bone marrow or peripheral stem cell transplantation: a preliminary study

Bone Marrow Transplantation volume 30, pages 485–490 (2002)Cite this article

Abstract

Pulmonary complications pose a major clinical problem after bone marrow transplantation (BMT) or peripheral blood stem cell transplantation (PBSCT). The underlying pathophysiological mechanisms are under investigation. Twenty patients with infectious or non-infectious pulmonary complications after BMT or PBSCT underwent bronchoalveolar lavage (BAL) and tumor necrosis factor-α (TNFα), interleukin-10 (IL-10) and interleukin-18 (IL-18) mRNA expression was determined in BAL cells in comparison to 11 healthy volunteers. Patients were divided into two groups (infectious pneumonia, n = 14 or idiopathic pneumonia syndrome (IPS)/bronchiolitis obliterans (BO), n = 6). TNFα expression was significantly increased in both patient groups compared to the control (0.40 ± 0.07 and 0.39 ± 0.09 vs 0.15 ± 0.05; P < 0.05; semiquantitative PCR analysis; mean ± s.e.m.). IL-10 expression was significantly elevated the group of patients with infectious pneumonia in comparison to normal controls (0.15 ± 0.06 vs 0.01 ± 0.01; P < 0.05) but not in patients with IPS/BO (0.05 ± 0.04; P > 0.05). However, IL-10 was not expressed in BAL cells of all patients and control individuals. IL-18 expression was significantly decreased in the both patient groups (1.47 ± 0.24 and 1.79 ± 0.63) in comparison to the control group (4.29 ± 0.77; P < 0.05). Survival analysis showed a significant association between elevation of TNFα and poor prognosis (P < 0.05). These results highlight the immunoregulatory disturbances in the lungs after BMT/PBSCT.

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Authors and Affiliations

  1. Department of Internal Medicine, Pulmonology, University Hospital Eppendorf, Germany

    H-P Hauber, A Mikkilä, JM Erich, A Meyer & A Pforte

  2. Center for Bone Marrow Transplantation, University Hospital Eppendorf, Germany

    N Kröger & AR Zander

  3. Institute for Medical Statistics, University Hospital Eppendorf, Germany

    V Schoder

Authors
  1. H-P Hauber
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  2. A Mikkilä
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  3. JM Erich
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  5. A Meyer
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  7. AR Zander
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Hauber, HP., Mikkilä, A., Erich, J. et al. TNFα, interleukin-10 and interleukin-18 expression in cells of the bronchoalveolar lavage in patients with pulmonary complications following bone marrow or peripheral stem cell transplantation: a preliminary study. Bone Marrow Transplant 30, 485–490 (2002). https://doi.org/10.1038/sj.bmt.1703722

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