Abstract
THE proto-oncogene c-jun is a component of the AP-1 transcription factor family involved in the mediation of nuclear events elicited by extracellular stimuli1–3. The c-jun protein is negatively regulated by phosphorylation of residues near the carboxy terminus which are dephosphorylated in response to phorbol esters4. Here we identify two serine residues in the amino terminal Al transactivation domain which are phosphorylated in response to a variety of mitogens, phorbol esters and activated ras (ref. 5). We present evidence that mitogen-activated protein-serine (MAP) kinases (pp54 and pp42/44) specifically phosphorylate these sites and that their phosphorylation positively regulates the transacting activity of c-jun. The MAP kinase enzymes pp54 and pp42/44 are regulated by tyrosine as well as serine/threonine phosphorylation6,7. MAP kinase activation of c-jun may underlie the common stimulation of this transcription factor by mitogens, growth factors and oncogenes.
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References
Bohmann, D. et al. Science 238, 1386–1392 (1987).
Angel, P. et al. Nature 332, 166–171 (1988).
Woodgett, J. R. Semin. Cancer Biol. 4, 389–397 (1990).
Boyle, W. J. et al. Cell 64, 573–584 (1991).
Binetruy, B., Smeal, T. & Karin, M. Nature 351, 122–127 (1991).
Anderson, N. G., Maller, J. L., Tonks, N. K. & Sturgill, T. W. Nature 343, 651–653 (1990).
Kyriakis, J. M., & Avruch, J. J. biol. Chem. 265, 17355–17363 (1990).
Sturgill, T. W., Ray, L. B., Erikson, E. & Maller, J. L. Nature 334, 715–718 (1988).
Boulton, T. G. et al. Cell 65, 663–675 (1991).
Kyriakis, J. M. & Avruch, J. FASEB J. 5, A797 (1991).
Kyriakis, J. M., Brautigan, D. L., Ingebritsen, T. S. & Avruch, J. J. biol. Chem. 266, 10043–10046 (1991).
Payne, D. M. et al. EMBO J. 10, 885–892 (1991).
Schanen-King, C., Nel, A., Williams, L. K. & Landreth, G. Neuron 6, 915–922 (1991).
Vicendo, P., Fauvel, J., Ragab-Thomas, J. M. F. & Chap, H. Biochem. J. 278, 435–440 (1991).
Bohmann, D. & Tjian, R. Cell 59, 709–717 (1989).
Baichwal, V. R. & Tjian, R. Cell 63, 815–825 (1990).
Baichwal, V. R., Park, A. & Tjian, R. Nature 352, 165–168 (1991).
Studier, F. W., Rosenburg, A. H., Dunn, J. J. & Dubendorff, J. W. Meth. Enzym. 185, 60–89 (1990).
Darrow, A. L., Rickles, R. J. & Strickland, S. Meth. Enzym. 190, 110–119 (1990).
Chiu, R. et al. Cell 54, 541–552 (1988).
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Pulverer, B., Kyriakis, J., Avruch, J. et al. Phosphorylation of c-jun mediated by MAP kinases. Nature 353, 670–674 (1991). https://doi.org/10.1038/353670a0
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DOI: https://doi.org/10.1038/353670a0
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