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World Health Organisation – supervised interlaboratory comparison of ELISAs for the serological detection of Salmonella enterica serotype Enteritidis in chickens

Published online by Cambridge University Press:  15 May 2009

P. A. Barrow ,
M. Desmidt ,
R. Ducatelle ,
M. Guittet ,
H. M. J. F. van der Heijden ,
P. S. Holt ,
J. H. J. Huis in't Velt ,
P. McDonough ,
K. V. Nagaraja  and
R. E. Porter
...Show all authors
P. A. Barrow
Affiliation:
Institute for Animal Health, Compton Laboratory, Compton, Nr. Newbury, Berkshire, RG20 7NN, UK
M. Desmidt
Affiliation:
Faculty of Veterinary Medicine, University of Gent, Casinoplein 24, B-9000, Gent Belgium
R. Ducatelle
Affiliation:
Faculty of Veterinary Medicine, University of Gent, Casinoplein 24, B-9000, Gent Belgium
M. Guittet
Affiliation:
Laboratoire Central de Recherches Avicole et Porcine, Centre Nationale d'Etudes Veterinaires et Alimentaires (CNEVA), Beaucemaine, BP53, 22440 Ploufragan, France
H. M. J. F. van der Heijden
Affiliation:
Stichting Gezondheidsdienst voor Dieren in Zuid Nederland, Postbus 4, 5280 AA Boxtel, The Netherlands
P. S. Holt
Affiliation:
USDA Southeast Poultry Research Laboratory, PO Box 5657, Athens, Georgia 30604, USA
J. H. J. Huis in't Velt
Affiliation:
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
P. McDonough
Affiliation:
Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, PO Box 5786, Ithaca, New York 14853, USA
K. V. Nagaraja
Affiliation:
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, St Paul, Minnesota 55108, USA
R. E. Porter
Affiliation:
Animal Disease Diagnostic Laboratory, Purdue University, 1175 VADL, West Lafayette, Indiana 47907–1175, USA
K. Proux
Affiliation:
Laboratoire Central de Recherches Avicole et Porcine, Centre Nationale d'Etudes Veterinaires et Alimentaires (CNEVA), Beaucemaine, BP53, 22440 Ploufragan, France
F. Sisak
Affiliation:
Veterinary Research Institute, Hudcova 70, 621-32 Brno, Czech Republic
C. Staak
Affiliation:
Bunderinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Postfach 33 00 13, D-14191, Berlin 33, Germany
G. Steinbach
Affiliation:
Bunderinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Bereich Jena, D-07722 Jena, Germany
C. J. Thorns
Affiliation:
Central Veterinary Laboratory, New Haw, Weybridge, Surrey, KT15 3NB, UK
C. Wray
Affiliation:
Central Veterinary Laboratory, New Haw, Weybridge, Surrey, KT15 3NB, UK
F. van Zijderveld
Affiliation:
Central Veterinary Institute (CD-DLO), PO Box 65, NL-8200 AB, Lelystad, The Netherlands
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A collaborative exercise, supervised by the World Health Organisation, was set up to compare ELISAs used for the serological detection of Salmonella enteritica serotype Enteritidis in chickens. The aim was to ascertain how far agreement could be reached on the interpretation of optical density readings for high titre, intermediate titre and low titre sera. Two sets of sera were sent to 14 participants. The first set compared high, medium and low titre sera raised in specified-pathogen-free and commercial broiler breeder chickens. The second set comprised 20 sera of different antibody titres raised in commercial birds reared under laboratory conditions and sent blind. Both indirect and double-antibody sandwich blocking ELISAs were used with a number of different detecting antigens. With a few exceptions good agreement was reached on the interpretation of results obtained from high and low titre sera from the optical density obtained with a single serum dilution. Differences were observed in the interpretation of medium titre sera. The results suggested that most ELISAs produce reasonably comparable results and that practical problems may arise from interpretation of the results mainly as a result of the choice of the criteria used for differentiating sera obtained from infected and uninfected chickens. These problems are discussed.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 1 , August 1996 , pp. 69 - 77
Copyright
Copyright © Cambridge University Press 1996

References

1.Rodrigue, DC, Tauxe, RV, Rowe, B.International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect 1990; 105: 21–7.CrossRefGoogle ScholarPubMed
2. Statutory Instruments. The poultry breeding flocks and hatcheries (Registration and Testing) Order no. 1963. London: HMSO, 1989.Google Scholar
3.Council Directive 92/117/EEC of 17 December 1992 concerning measures against specified zoonoses and specified zoonotic agents in animals and products of animal origin in order to prevent outbreaks of foodborne infections and intoxications. Of J Europ Communities 1993; L62: 38–48.Google Scholar
4.Barrow, PA, ELISAs and the serological analysis of salmonella infections in poultry: a review. Epidemiol Infect 1992; 109: 361–9.CrossRefGoogle ScholarPubMed
5. Report. Unpublished report of the World Health Organisation informal meeting on enzyme immunoassay for brucellosis management.Nepean, Ontario, Canada,29–30 May 1991. VPH/89.84. Geneva:World Health Organisation, 1989.Google Scholar
6. Report. Unpublished report of the World Health Organisational informal consultation on enzyme immunoassay for brucellosis diagnosis and research.Geneva,5–6 April 1990. VPH/90.90. Geneva:World Health Organisation, 1990.Google Scholar
7.Hassan, JO, Barrow, PA, Mockett, APA, Mcleod, S.Antibody response to experimental Salmonella typhimurium infection in chickens measured by ELISA. Vet Rec 1990; 126: 519–22.Google ScholarPubMed
8.Nicholas, RAJ, Cullen, GA.Development of an ELISA for detecting antibodies to Salmonella enteritidis in chicken flocks. Vet Rec 1991; 128: 74–6.CrossRefGoogle ScholarPubMed
9.Barrow, PA, Lovell, MA.Experimental infection of egg-laying hens with Salmonella enteritidis. Avian Pathol 1991; 20: 339–52.Google ScholarPubMed
10.Timoney, JF, Sikora, N, Shivaprasad, HL, Opitz, M.Detection of antibody to Salmonella enteritidis by a gm flagellin-based ELISA. Vet Rec 1990; 127: 168–9.Google Scholar
11.Baay, MFD, Huis in't Veld, JHJ.Alternative antigens reduce cross-reactions in an ELISA for the detection of Salmonella enteritidis in poultry. J Appl Bact 1993; 74: 243–7.CrossRefGoogle Scholar
12.Thorns, CJ, Sojka, MG, Chasey, D.Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody. J Clin Microbiol 1990; 28: 2409–14.CrossRefGoogle ScholarPubMed
13.Kim, CJ, Nagaraja, KV, Pomeroy, BS.Enzyme-linked immunosorbent assay for the detection of Salmonella enteritis infections in chickens. Amer J Vet Res 1991; 52: 1069–74.CrossRefGoogle Scholar
14.Van Zijderveld, FG, Van Zijderveld-van Bemmel, AM, Anakotta, J.Comparison of four different enzyme-linked immunosorbent assays for serological diagnosis of Salmonella enteritidis infections in experimentally infected chickens. J Clin Microbiol 1992; 30: 2560–6.CrossRefGoogle ScholarPubMed
15.Smith, HW, Tucker, JF.The effect of antibiotic therapy on the faecal excretion of Salmonella typhimurium by experimentally infected chickens. J Hyg 1975; 75: 275–92.CrossRefGoogle Scholar
16.Mcleod, S, Barrow, PA.Lipopolysaccharide-specific IgG in egg yolk from two chicken flocks infected with Salmonella enteritidis. Lett Appl Microbiol 1991; 13: 294–7.CrossRefGoogle Scholar
17.Dilling, GW, Smith, BP.International standardisation of ELISA for serologic detection of Salmonella dublin carrier cows. Proceedings of the International Symposium on Salmonella and Salmonellosis. Ploufragan, France 1992; 87–8.Google Scholar
18.Barrow, PA.Use of ELISAs for monitoring Salmonella in poultry. Vet Rec 1994; 134: 99.CrossRefGoogle ScholarPubMed
19.Barrow, PA, Wray, C, eds. Proceedings of the European Commission Workshop on ELISAs for serological diagnosis of Salmonella infection in poultry.Brussels,7–9 June 1993.Directorate General for Agriculture, Commission of the European Communities,Brussels, 1994.Google Scholar
20.Van der Heijden, HMJF.Effect of periodate oxidation of LPS on the specificity of Salmonella-ELISAs in poultry. Proceedings of the European Commission Workshop on ELISAs for serological diagnosis of Salmonella infection in poultry,Brussels,7–9 June 1993.Directorate General for Agriculture, Commission of the European Communities,Brussels, 1994.Google Scholar
21.Huis in't Veld, JHJ, van Asten, AJAM, Baay, MFD, Kusters, JG, van der Zeijst, BAM.Selection of alternative antigens for the development of a specific ELISA for the detection of Salmonella enteritidis in poultry. Proceedings of the European Commission Workshop on ELISAs for serological diagnosis of Salmonella infection in poultry,Brussels,7–9 June 1993.Directorate General for Agriculture, Commission of the European Communities,Brussels, 1994.Google Scholar