Mechanisms of asthma and allergic inflammationAdiponectin attenuates allergen-induced airway inflammation and hyperresponsiveness in mice
Section snippets
Allergen sensitization and challenge
This study was approved by the Harvard Medical Area Standing Committee on Animals. Male and female 4-week-old BALB/cJ mice were purchased from The Jackson Laboratory (Bar Harbor, Me). Mice were sensitized to chicken egg albumin (ovalbumin [OVA]; Grade V, Sigma-Aldrich, St Louis, Mo) on day 0 by means of an intraperitoneal injection of 20 Ī¼g of OVA and adjuvant and 2 mg of aluminum hydroxide (Al[OH]3; J. T. Baker, Phillipsburg, NJ) dispersed in 0.2 mL of PBS, as previously described.44 The mice
Body weight
There was a small reduction in body weight the day after implantation of the mini-Alzet pumps that averaged 2.3% Ā± 0.6% of the initial body weight. Body weight recovered to baseline values by the day of the first OVA/PBS aerosol challenge in both TBS- and adiponectin-treated mice. There was no significant effect of either OVA/PBS challenge or adiponectin versus TBS treatment on body weight.
BAL cells and cytokines
Compared with PBS challenge, OVA challenge caused a significant increase in BAL macrophages (P < .05),
Discussion
Our results indicate for the first time that treatment of mice with full-length adiponectin at a dose sufficient to induce an approximate 60% increase in serum adiponectin levels virtually abolishes OVA-induced changes in inflammatory cell influx (Fig 1) and TH2 cytokine expression in the lungs (Fig 2), as well as OVA-induced airway hyperresponsiveness (Fig 3). Obesity is associated with decreases in serum adiponectin levels that are on the same order of magnitude as the increase in serum
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Supported by National Institutes of Health grants HL33009 and HL077499, National Institute of Environmental Health Sciences grants ES013307 and ES00002, a generous gift from Paul and Mary Finnegan, and a Charles H. Hood Foundation grant.
Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest.