Food allergy, dermatologic diseases, and anaphylaxisCharacterization of FcεRI-bearing CD123+ blood dendritic cell antigen-2+ plasmacytoid dendritic cells in atopic dermatitis☆
Section snippets
Methods
For the section “Reagents,” please see the ancillary material in the Journal's Online Repository at www.mosby.com/jaci.
CD123+BDCA-2+ pDCs in the peripheral blood of patients with AD display different phenotypical characteristics
First, we evaluated phenotypical characteristics of CD123+BDCA-2+ pDCs in the peripheral blood of patients with AD and healthy, nonatopic volunteers in detail by flow cytometry. BDCA-2+CD123+ pDCs freshly isolated from the blood of patients with AD expressed higher amounts of the costimatory molecule CD80 and the MHC I molecule on their cell surface than BDCA-2+CD123+ pDCs of healthy, nonatopic volunteers (Fig 1). In contrast, surface expression of L-selectin CD62L and cutaneous lymphocyte
Discussion
Although it is generally agreed that pDCs are involved in the elicitation of TH2-type immune responses, the exact mechanisms underlying this process have not been elucidated so far.1 Here we show for the first time that CD123+BDCA-2+ pDCs in the peripheral blood of patients with AD express enhanced amounts of the trimeric variant of FcεRI, which, similar to myeloid DCs, consists of the IgE-binding α-chain and the γ-chain dimer and is mostly occupied with IgE molecules.18 In contrast with the
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Supported by grants from the Deutsche Forschungsgemeinschaft (DFG NO/454-1-1 and DFG NO/454-2-1) and Bonner Forschungsförderung and by the grant Y078GEN of the Austrian Science Fund and the Forschungsgzentrum für Molekulare Medizin project of the Austrian Academy of Science.