Brief ReportSleep Loss Activates Cellular Inflammatory Signaling
Section snippets
Methods and Materials
Fourteen subjects (seven men and seven women) who were medically healthy, as determined by medical history, physical examination, and laboratory testing, were recruited between October 2006 and June 2007. All subjects fulfilled criteria for Never Mentally Ill as determined by Structured Clinical Interview for Diagnostic and Statistical Manual—IV (SCID) (mean age = 51.8 years [SD = 12.8]; 50% women; mean education level = 15.8 years [SD = 4.6]; mean body mass index [BMI] = 26.9 [SD = 3.6]; 35.7%
Results
Subjects displayed an increase of PBMC NF-κB activation after PSD [condition effect: F(2,82.0) = 7.0, p < .01; Figure 1A]. Pairwise comparisons showed that levels of NF-κB activation were markedly increased the morning after PSD compared with baseline- and recovery morning levels (−.054, 95% confidence interval −.099 to −.009 ng NF-κB protein/μg total nuclear protein, p < .01; .057, .019–.156, 95% confidence interval, p < .01), with a 30% increase relative to baseline levels. Levels of NF-κB
Discussion
Here we present evidence that acute sleep loss induces a rapid increase in activation of the transcription factor NF-κB in PBMC, providing a potential molecular mechanism for the effects of sleep loss on proinflammatory gene expression and circulating levels of inflammatory mediators (7, 8, 10). This observation that sleep loss results in nuclear translocation of NF-κB verifies previous bioinformatic indications (10) and closes an important gap in understanding the cellular mechanisms by which
References (20)
- et al.
The metabolic consequences of sleep deprivation
Sleep Med Rev
(2007) - et al.
Soluble TNF-alpha receptor 1 and IL-6 plasma levels in humans subjected to the sleep deprivation model of spaceflight
J Allergy Clin Immunol
(2001) - et al.
Effect of sleep loss on C-reactive protein, an inflammatory marker of cardiovascular risk
J Am Coll Cardiol
(2004) - et al.
Nuclear receptors versus inflammation: Mechanisms of transrepression
Trends Endocrinol Metab
(2006) - et al.
Stress-induced enhancement of NF-kappaB DNA-binding in the peripheral blood leukocyte pool: Effects of lymphocyte redistribution
Brain Behav Immun
(2004) - et al.
Nocturnal proinflammatory cytokine-associated sleep disturbances in abstinent African American alcoholics
Brain Behav Immun
(2004) - et al.
Healthy older adults' sleep predicts all-cause mortality at 4 to 19 years of follow-up
Psychosom Med
(2003) - et al.
Does insomnia kill?
Sleep
(2005) - et al.
Cardiovascular disease, interleukin-6, and risk of mortality in older women: The women's health and aging study
Circulation
(2001) - et al.
NF-kappaB: linking inflammation and immunity to cancer development and progression
Nat Rev Immunol
(2005)