Nano titanium dioxide photocatalytic protein tyrosine nitration: A potential hazard of TiO2 on skin
Section snippets
Materials and methods
Materials. Three nano titanium dioxide (TiO2) powders in the forms of anatase, rutile (Zhejiang Hongsheng Material Technology Co., Ltd.) and Degussa P25 (P25, anatase/rutile, Degussa company, Germany), were commercially available and used without further treatment (Table 1). Bovine serum albumin (BSA) and rabbit polyclonal antibody against 3-nitrotyrosine were purchased from Sigma. All solvents and other reagents were the highest purity commercially available. High voltage mercury lamp (a
-photocatalytic protein tyrosine nitration
As shown in Fig. 1A and B, protein tyrosine nitration was generated in the UV-irradiated reaction mixture containing 0.5 mg/ml BSA, 1.67 mg/ml TiO2 and 1 mM , which was detected by spectrophotometer (Fig. 1A) and Western blotting (Fig. 1B), respectively. There was no 3-nitrotyrosine formation from the treatment with TiO2 or alone, which demonstrated that TiO2 and were both essential for the photo-nitration. The production of 3-nitrotyrosine increased with prolonging the irradiation
Acknowledgments
This research was supported by the National Basic Research Program (973 Program) of China (No. 2006CB705606), the National Natural Science Foundation of China (No. 30670481), the Program for New Century Excellent Talents in University (No. NCET-05-0649), and E-Institutes of Shanghai Municipal Education Commission (No. E-04010).
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These authors contributed equally to this work.