Original ArticlesTransforming growth factor-beta (TGF-β1) genotype and lung allograft fibrosis
Section snippets
Patients and specimens
Biopsy specimens from 91 consecutive lung transplant performed in the Wythenshawe Lung Transplant Program between January 1990 and March 1996 were analysed. Patient demographics and surgical procedures are summarised in Table I. A total of 780 transbronchial biopsies from all the recipients were reviewed to classify and score rejection, infection and lung fibrosis. For genotype analysis a blood sample was obtained from each patient and, for comparison, blood samples were collected from 96
TGF-β1 gene polymorphism in patients and normal controls
Patients’ demographics, mortality and type of procedure are summarised in Table 1. We have identified 2 polymorphisms in the leader sequence of TGF-β1 gene, located at codon 10 (amino acid +10) and codon 25 (amino acid +25). At codon 10 there are two alternatives either leucine (L) and proline (P). The distribution of codon 10 alleles was similar in the lung transplant recipients regardless of pretransplant pathology, with the exception of the cystic fibrosis patients who showed a trend towards
Discussion
Lung allografts are usually exposed to multiple injuries as a result of ischaemia and reperfusion, acute rejection or infection, which may damage either pulmonary epithelium or capillary endothelium. Lesions are focal and homogeneous or generalised in distribution. Leakage of serum proteins, inflammatory cells and platelets into the interstitial and alveolar space is apparent several hours after injury.11 Marked increases in total DNA content soon after injury reflect dramatic increases in
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