Atopic asthma and TNF-308 alleles: linkage disequilibrium and association analyses
Section snippets
Abbreviations
- TNF
tumor necrosis factor
- HLA
human leukocyte antigen
- ST
prick skin test
- BBS
borate buffered saline
- TDT
transmission disequilibrium test
- DARIA
double antibody radioimmunoassay
Study population
The study population (239 patients) has been previously described [5]. Participants were analyzed by ST for reactivity towards 18 glycerinated extracts of common inhalant allergens (Hollister-Stier Laboratories, Spokane, WA, USA). ST positivity was defined by a wheal diameter > 3 mm more than the negative control. Atopy was defined as ST positivity to at least one of the allergenic extract tested. As the results of tests performed with P. judaica and P. officinalis pollen extracts were in each
Association of TNF-308 alleles an TNF/HLA-DRB1* haplotypes to ST reactivity to D. Pteronyssinus, Lolium Perenne, and Parietaria
Atopics were subdivided according to ST reactivity to D. pteronyssinus, Lolium perenne, and Parietaria and the association between these phenotypes, TNF alleles, and selected TNF/HLA-DRB1* haplotypes was investigated (Table 2).
No association of TNF-308 alleles with responsiveness to specific allergens was observed. The association of HLA-DRB1*03 to responsiveness to Lolium allergens has been reported by other groups [7] . The positive and negative association of ST positivity to Parietaria to
Discussion
The results of this study indicate that TNF2 is transmitted to asthmatics with the DRB1* alleles associated with atopy, and suggests that haplotypes TNF2/DRB1*03 and TNF2/DRB1*1104 contain functional alleles controlling asthma and atopy to Lolium and Parietaria, respectively. The modest association of asthma to TNF2 observed in the parents subgroup appears to be secondary to association to TNF2/DRB1*1104 (Table 3). These observations do not support the hypothesis that HLA region genes control
Acknowledgements
This study is supported by Telethon, Italy (grant E487).
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