Elsevier

Experimental Gerontology

Volume 29, Issue 2, March–April 1994, Pages 187-195
Experimental Gerontology

Aging effects on hepatic NADPH cytochrome P450 reductase, CYP2B1 & 2, and polymeric immunoglobulin receptor mRNAs in male fischer 344 rats

https://doi.org/10.1016/0531-5565(94)90050-7Get rights and content

Abstract

Aging perturbs the expression of many liver proteins, but the mechanisms remain unresolved. Expression of hepatic NADPH cytochrome P450 reductase, phenobarbital-induced CYP2B1 & 2, and the polymeric immunoglobulin receptor (pIgR) decline as a function of aging. We examined the effect of aging on the expression of the mRNA transcripts of these proteins,as well as those of α2u-globulin and β-actin in male F344 rats. Despite age-related losses in the expression of P450 reductase and plasma membrane-bound pIgR in the rat liver (≈30–50%), aging is accompanied by 1) no change and 2) a modest decline (<20%) in their respective mRNA steady state levels. On the other hand, the expression of phenobarbital-induced microsomal CYP2B1 & 2 and the steady state level of its mRNA exhibit parallel age-dependent shifts. The mRNA transcript for α2u-globulin declines between maturity and old age, whereas the β-actin mRNA level remains unchanged. These preliminary data are consistent with previous studies which suggest that aging may perturb hepatic CYP2B1 & 2 and α2u-globulin at the transcriptional level, whereas changes in the expression of P450 reductase and pIgR may reflect posttranscriptional modifications.

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