Elsevier

Methods in Enzymology

Volume 186, 1990, Pages 660-665
Methods in Enzymology

[71] Oxy radicals in endotoxin shock

https://doi.org/10.1016/0076-6879(90)86163-PGet rights and content

Publisher Summary

There is considerable indirect evidence supporting the role of oxygen radicals in circulatory shock. Endotoxin is a toxin released from dead gram-negative bacteria, and this toxin produces circulatory depression in septicemic patients concomitantly with a variety of other cellular dysfunctions. This chapter discusses the role of oxy radicals in endotoxin shock. Polymorphonuclear leukocytes (PMN)-depleted rats are used to examine the effect of PMNs on experimental shock. Anti-rat PMN antibody is obtained by immunizing rabbits with rat PMNs in Freund's complete adjuvant, using the methods of Ward and Cochrane. The PMN preparation is obtained by instilling 0.12% oyster glycogen type II intraperitoneally into rats, followed by peritoneal lavage with sterile saline 6 hours later. Antirat PMN antibody is injected intraperitoneally into the rats to produce PMN-depleted rats. The number of PMNs is significantly reduced at 18 hours after injection of antibody. The reduction of systolic blood pressure and the increase in serum lysosomal enzymes induced by the injection with endotoxin are significantly inhibited by the pretreatment with anti-rat PMN antibody. The chapter also demonstrates that superoxide radicals and catalase effectively protects the aggravation of shock induced by endotoxin. This is a very important observation that suggests that superoxide and hydrogen peroxide exert certain effects on the experimental shock state.

References (7)

  • B. Gray

    Toxicol. Appl. Pharmacol.

    (1981)
  • T. Yoshikawa et al.

    Bioelectrochem. Bioenerg.

    (1987)
  • T. Yoshikawa et al.

    J. Clin. Biochem. Nutr.

    (1986)
There are more references available in the full text version of this article.

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