A simple and sensitive method for the quantitative estimation of collagen
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Boric acid and Molybdenum trioxide synergistically stimulate osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells
2024, Journal of Trace Elements in Medicine and BiologyAntifibrotic effect of brown algae-derived fucoidans on osteoarthritic fibroblast-like synoviocytes
2022, Carbohydrate PolymersMolybdenum trioxide enhances viability, osteogenic differentiation and extracellular matrix formation of human bone marrow-derived mesenchymal stromal cells
2021, Journal of Trace Elements in Medicine and BiologyCitation Excerpt :Expression of COL1A1, a relevant marker gene for ECM formation [40], was diminished in a concentration-dependent manner in MoO3 groups on D7 and only a slight upregulation above the control’s level was observed thereafter. Nevertheless, the actual amount of collagen produced and deposited by BMSCs, measured via Sirius Red staining [29–31], was found to be significantly increased in presence of 1 and 2 mM MoO3. Possible explanations for the distinct differences between gene expression levels and the proteins’ actual production are discussed controversially.
Genistein inhibits chondrogenic differentiation and mineralization of ATDC5 cells
2021, Biochemical and Biophysical Research CommunicationsCitation Excerpt :The absorbance of the elution was detected at 650 nm using a microplate reader (Vmax, Molecular Devices, CA, USA). Collagen fibers were detected by the SR method [27]. The cells were fixed with 4% paraformaldehyde for 30 min at 4 °C and stained with 0.1% Sirius Red F3B in a saturated aqueous solution of picric acid for 1 h.