A modified uronic acid carbazole reaction

doi:10.1016/0003-2697(62)90095-7

Analytical Biochemistry

Volume 4, Issue 4, October 1962, Pages 330-334

https://doi.org/10.1016/0003-2697(62)90095-7 Get rights and content

Abstract

A modification of Dische's carbazole reaction for uronic acid in the presence of borate is described. The advantages of the procedure are:

1.
(1) There is an approximately twofold increase of sensitivity. The OD is a linear function of concentration between 4 and 40 μg/ml.
2.
(2) Maximum color develops immediately.
3.
(3) The color is stable for at least 16 hr.
4.
(4) There is greater reproducibility and reduction of interference by chloride ion and oxidants.

It has been found possible to distinguish between heparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures.

References (15)

Z. Dische
J. Biol. Chem
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S. Schiller
Biochim. et Biophys. Acta
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S. Gurin et al.
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F.B. Seibert et al.
J. Biol. Chem
(1946)
J.D. Gregory
Arch. Biochem. Biophys
(1960)
Z. Dische
Biochem. Z
(1927)
N.R. Ringertz
Acta Chem. Scand
(1960)

There are more references available in the full text version of this article.

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¹: Supported by grants from the Empire Rheumatism Council and the Pearl Insurance Company.

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A modified uronic acid carbazole reaction

Abstract

J. Biol. Chem

Biochim. et Biophys. Acta

J. Biol. Chem

J. Biol. Chem

Arch. Biochem. Biophys

Biochem. Z

Acta Chem. Scand