Reduction of myocardial infarct size by neutrophil depletion: Effect of duration of occlusion

doi:10.1016/0002-8703(86)90461-8

American Heart Journal

Volume 112, Issue 4, October 1986, Pages 682-690

https://doi.org/10.1016/0002-8703(86)90461-8 Get rights and content

Abstract

Experiments were performed in the dog to examine the effects of neutropenia on ultimate infarct size resulting from short (90 minutes) or prolonged (4 hours) circumflex coronary artery occlusion. Sheep antiserum to canine neutrophils was used to produce neutropenia. Control animals received nonimmune serum. Neutrophil infiltration into myocardial infarcts was examined using histopathologic techniques and a semiquantitative scoring system. In 90-minute occlusions with 24-hour reperfusion, neutropenia was associated with the development of significantly smaller infarcts: normopenic group, 43.2% ± 3.3% (n = 7) vs. neutropenic group, 26.6% ± 3.7% (n = 10) of the area at risk, $X$ ± SEM. However, in 4-hour occlusion with 6-hour reperfusion experiments, the tendency of neutrophil depletion to reduce infarct size did not reach statistical significance (46.4% ± 7.2% vs. 31.5% ± 6.0% of the area at risk, normopenic vs. neutropenic) despite differences in neutrophil infiltration into the reperfused region. The observed differences in ultimate infarct size could not be attributed to differences in myocardial oxygen consumption. The results suggest that a significant amount of myocardial infarction induced by a limited duration of coronary artery occlusion followed by reperfusion is neutrophil dependent and appears to be less important in determining the fate of myocardium subjected to more prolonged periods of ischemia followed by reperfusion.

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^☆: Supported by grants from the National Institutes of Health (HL-19782-06) and the American Heart Association of Michigan.

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Reduction of myocardial infarct size by neutrophil depletion: Effect of duration of occlusion☆

Abstract

Am Heart J

J Surg Res

J Immunol Methods

Am J Cardiol

J Mol Cell Cardiol

Am J Cardiol

Am J Cardiol

Reduction of the extent of ischemic myocardial injury by neutrophil depletion in the dog

Circulation

The effect of ibuprofen on accumulation of 111Indium-labelled platelets and leukocytes in experimental myocardial infarction

Circulation

Role of leukocytes in acute myocardial infarction in anesthetized dogs: relationship to salvage by antiinflammatory drugs

J Pharmacol Exp Ther

Ibuprofen inhibits granulocyte responses to inflammatory mediators, a proposed mechanism for reduction of experimental myocardial infarct size

Inflammation

Reduction by antineutrophil serum of experimental infarct size

Clin Res

Ischemic changes in the canine heart as affected by the dimethyl analogue of propranolol UM-272 (SC-27761)

J Pharmacol Exp Ther

Technique for precise graded arterial stenosis and occlusion

Basic Res Cardiol

Platelet depletion in experimental myocardial infarction

Basic Res Cardiol

Effects of diltiazem on extent of ultimate myocardial injury resulting from temporary coronary artery occlusion in dogs

J Cardiovasc Pharmacol

Multiple range and multiple f-tests

Biometrics

The effect of ibuprofen on accumulation of ¹¹¹Indium-labelled platelets and leukocytes in experimental myocardial infarction