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Magnetized Aerosols Comprising Superparamagnetic Iron Oxide Nanoparticles Improve Targeted Drug and Gene Delivery to the Lung

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ABSTRACT

Purpose

Targeted delivery of aerosols could not only improve efficacy of inhaled drugs but also reduce side effects resulting from their accumulation in healthy tissue. Here we investigated the impact of magnetized aerosols on model drug accumulation and transgene expression in magnetically targeted lung regions of unanesthetized mice.

Methods

Solutions containing superparamagnetic iron oxide nanoparticles (SPIONs) and model drugs (fluorescein or complexed plasmid DNA) were nebulized to unanesthetized mice under the influence of an external magnetic gradient directed to the lungs. Drug accumulation and transgene expression was subsequently measured at different time points.

Results

We could demonstrate 2–3 fold higher accumulation of the model drug fluorescein and specific transgene expression in lung regions of mice which had been exposed to an external magnetic gradient during nebulization compared to the control mice without any exposure to magnetic gradient.

Conclusions

Magnetized aerosols present themselves as an efficient approach for targeted pulmonary delivery of drugs and gene therapeutic agents in order to treat localized diseases of the deeper airways.

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ACKNOWLEDGMENTS & DISCLOSURES

This work was supported by the BMBF BioFuture programme (FKZ0311898), the programme 13 N9182, LMUexcellent (Investitionsfonds), DFG RU 911/9-1 and Emma Thaler Foundation. Financial support by the German Research Foundation, through DFG Research Unit FOR917 (Project PL 281/3-1) and the German Federal Ministry of Education and Research through grant ELA 10/002 the German Excellence Cluster m4 ‘Nanosystems Initiative Munich’ awarded to Olga Mykhaylyk is greatfully acknowledged. We also thank Dr. W. Förster and Dr. T. Budiman (both TSE Systems, Bad Homburg, Germany) for technical and financial support of NOIS.

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Authors and Affiliations

  1. Department of Pediatrics, Ludwig-Maximilians-University, Lindwurmstr. 2a, 80337, Munich, Germany

    Guenther Hasenpusch, Johannes Geiger, Manish K. Aneja & Carsten Rudolph

  2. Institute of Pathology, Ludwig-Maximilians-University Munich, 80337, Munich, Germany

    Kai Wagner

  3. Institute of Experimental Oncology, Klinikum Rechts der Isar, Technical University of Munich, 81675, Munich, Germany

    Olga Mykhaylyk

  4. Physikalisch-Technische Bundesanstalt, 10587, Berlin, Germany

    Frank Wiekhorst & Lutz Trahms

  5. IMETUM, Technical University of Munich, 84748, Garching, Germany

    Alexandra Heidsieck & Bernhard Gleich

  6. Chemicell GmbH, 10823, Berlin, Germany

    Christian Bergemann

Authors
  1. Guenther Hasenpusch
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  2. Johannes Geiger
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  3. Kai Wagner
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  4. Olga Mykhaylyk
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  5. Frank Wiekhorst
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  7. Alexandra Heidsieck
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  8. Bernhard Gleich
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  9. Christian Bergemann
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  10. Manish K. Aneja
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  11. Carsten Rudolph
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Corresponding author

Correspondence to Carsten Rudolph.

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Hasenpusch, G., Geiger, J., Wagner, K. et al. Magnetized Aerosols Comprising Superparamagnetic Iron Oxide Nanoparticles Improve Targeted Drug and Gene Delivery to the Lung. Pharm Res 29, 1308–1318 (2012). https://doi.org/10.1007/s11095-012-0682-z

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  • DOI: https://doi.org/10.1007/s11095-012-0682-z

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