Skip to main content
SpringerLink
Log in

Treatment with dehydroepiandrosterone (DHEA) stimulates oxidative energy metabolism in the liver mitochondria from developing rats

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Effects of treatment with DHEA (0.2 mg or 1.0 mg / kg body weight for 7 days) on oxidative energy metabolism on liver mitochondria from developing and young adult rats were examined. Treatment with DHEA resulted in a progressive dose-dependent increase in the liver weights of the developing animals without change in the body weight. In the young adult rats treatment with 1.0 mg DHEA showed increase only in the body weight. Treatment with DHEA stimulated state 3 and state 4~respiration rates in developing as well as young adult rats in dose-dependent manner with all the substrates used; magnitude of stimulation was age-dependent. In young adults the extent of simulation of state 3 respiration rates declined at higher dose (1.0~mg) of DHEA with glutamate and succinate as substrates. Stimulation of state 3 respiration rates was accompanied by increase in contents of cytochrome aa3, b and c + c1 and stimulation of ATPase and dehydrogenases activities in dose- and age-dependent manner.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ADP:

adenosine diphosphate

DCIP:

dicholrophenolindophenol

DHEA:

dehydroepiandrosterone

EDTAd:

disodium salt of ethylenediaminetetraacetic aci

MOPS:

4-morpholinopropanesulfonic acid

TMPD:

N,N,N',N'-tetra methyl-pphenylenediamine

References

  1. Milgrom E, Steroid hormone. In Hormones From molecues to disease: Baulieu EE and Kelly PA, (Ed.), Hermann Publishers and Hall. New York and London, pp. 387–438, 1990

    Google Scholar 

  2. Buvat J, Androgen therapy with dehydroepiandrosterone. World J Urol 21: 346–355, 2004

    Article  Google Scholar 

  3. Hinson JP, Raven PW, DHEA deficiency syndrome: a new term for old age? J Endocrinol 163: 1–5, 1999

    Article  PubMed  CAS  Google Scholar 

  4. Celec P, Starka L, Dehydroepiandrosterone—Is the fountain of fouth drying out? Physiol Res 52: 397–407, 2003

    PubMed  CAS  Google Scholar 

  5. Miller BC, Lau HW, Tyler NE, Cottam GL, Liver composition and lipid metabolism in NZB/W F1 female mice fed dehydroisoandrosterone. Biochem Biophys Acta 962: 25–36, 1988

    PubMed  CAS  Google Scholar 

  6. Cleary MP, Effect of dehydroisoandrosterone treatment on liver metabolism in rats. Int J Biochem 22: 205–210, 1990

    Article  PubMed  CAS  Google Scholar 

  7. McIntosh MK, Pan JS, Berdanier CD, In vitro studies on the effects of dehydroisoandrosterone and corticosterone on hepatic steroid receptor binding and mitochondrial respiration. Comp. Bichem Physiol Comp Physiol 104: 147–153, 1993

    Article  CAS  Google Scholar 

  8. Oliver TW, Clemens K, Action of dehydroepiandrosterone and its sulfate in the central nervous system: effects on cognition and emotion in animals and humans. Brain Res Rev 30: 264–288, 1999

    Article  Google Scholar 

  9. Natawa H, Toshihiko Y, Kiminobu G, Taijiro OKA, Mechanism of action of anti-aging DHEA-S and the replacement of DHEA-S. Mech Age Dev 123: 1101–1106, 2002

    Article  Google Scholar 

  10. Patel MA, Katyare SS, (2005) Effect of dehydroepiandrosterone (DHEA) treatment on oxidative energy metabolism in rat liver and brain mitochondria. A dose response study Arch Biochem Physiol, 2005 (Communicated)

  11. Katewa SD, Katyare SS, Treatment with antimalarials adversely affects the oxidative energy metabolism in rat liver mitochondria. Drug Chem Toxicol 27: 41–53, 2004

    Article  PubMed  CAS  Google Scholar 

  12. Pandya JD, Agarwal NA, Katyare SS, Effect of Dexamethasone treatment on oxidative energy metabolism in rat liver mitochondria during postnatal developmental periods. Drug Chem Toxicol 27: 389–403, 2004

    Article  PubMed  CAS  Google Scholar 

  13. Kaushal R., Dave KR, Katyare SS, Paracetamol hepatotoxicity and microsomal function. Environ Toxicol Pharmacol 7: 67–74, 1999

    Article  CAS  Google Scholar 

  14. Katyare SS, Satav JG, Impaired mitochondrial energy metabolism following paracetamol-induced hepatotoxicity in the rat. Br J Pharmacol 96: 51–58, 1989

    PubMed  CAS  Google Scholar 

  15. Ferreira J, Gil L, Nutriotional effects on mitochondrial bioenergetics: Alterations in oxidative phosphorylation by rat liver mitochondria. Biochem J 218: 61–67, 1984

    PubMed  CAS  Google Scholar 

  16. Katyare SS, Joshi MV, Fatterpaker P, Sreenivasan A, Effect of thyroid deficiency on oxidative phosphorylation in rat liver, kidney and brain mitochondria. Arch Biochem Biophys 182: 155–163, 1977

    Article  PubMed  CAS  Google Scholar 

  17. Chance B, Williams GR, The respiratory chain and oxidative phosphorylation. Nature 173: 1094–1095, 1954

    Article  Google Scholar 

  18. Subramaniam M, Katyare SS, Oxidative phosphorylation in mouse liver mitochondria during weaning. Mech Age Dev 54: 121–129, 1990

    Article  Google Scholar 

  19. Leighton F, Poole B, Beaufay H, Baudhuin P, Coffer JW, Flower S, de Duve C, The large scale separation of peroxisome, mitochondria and lysosomes from livers of rats injected with Triton X-100: Improved isolation procedures, analysis and biochemical properties of fractions. J Cell Biol 37: 482–513, 1968

    Article  PubMed  CAS  Google Scholar 

  20. Ochoa S, Malic dehydrogenase from pig heart, In: Colowick SP, Kaplan NO, (Ed), Methods Enzymol. vol. 1, Academic Press, New York, pp. 735–739, 1955

    Chapter  Google Scholar 

  21. King TE, Preparation of succinate-cytochrome c-reductase and the cytochrome b-c1 particle and the reconstitution of cytochrome c-reductase, In: Estabrook RW, Pullman ME, (Ed.), Methods Enzymol. , Academic Press, New York, pp. 216–225, 1967

    Google Scholar 

  22. Katewa SD, Katyare SS, A Simplified method for inorganic phosphate determination and its application for phosphate analysis in enzyme assays, Anal Biochem 323: 180–187, 2003

    Article  PubMed  CAS  Google Scholar 

  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, Protein measurement with Folin-phenol reagent. J Biol Chem 193: 265–275, 1951

    PubMed  CAS  Google Scholar 

  24. Jani MS, Telang SD, Katyare SS, Effect of corticosterone treatment on energy metabolism in rat liver mitochondria. J Steroid Biochem Mol Biol 38: 587–591, 1991

    Article  PubMed  CAS  Google Scholar 

  25. Katyare SS, Balasubramanian S, Parmar DV, Effect of corticosterone treatment on mitochondrial oxidative energy metabolism in developing rat brain. Exp Neurol 183: 241–248, 2003

    Article  PubMed  CAS  Google Scholar 

  26. Rajan RR, Katyare SS, . Is the first site of phosphoryrlation operative in rat brain mitochondria in early neonatal life: A critical reevaluation. Mech Age Dev 61:149-.161.1991

    Article  CAS  Google Scholar 

  27. Golovachev AF, Nadal'yak EA, Respiration and glycolysis in the liver of developing chick embryos and chicks. Zh Evol Biokhim Fiziol 11: 353–359, 1975

    PubMed  CAS  Google Scholar 

  28. Okada Y, Energy metabolism and neural activity in hippocampal slices of developing rat brain. No To Hattatsu 26: 113–118, 1994

    PubMed  CAS  Google Scholar 

  29. Rust RS, Energy metabolism of developing brain. Curr Opin Neurol 7: 160–165, 1994

    PubMed  CAS  Google Scholar 

  30. Poyton RO, Mc Ewen JE, Crosstalk between nuclear and mitochondrial genomes. Annu Rev Biochem 65: 563–607, 1996

    Article  PubMed  CAS  Google Scholar 

  31. Parker CR Jr, Dehydroepiandrosterone and dehydroepiandrosterone sulfate production in the human adrenal during development and aging. Steroids 64: 640–647, 1999

    Article  PubMed  CAS  Google Scholar 

  32. Steckelbroeck S, Watzka M, Lutjohall D, Makiola P, Nassen A, . Hans VH, Clusmann H, . Reissinger A, Ludwig M, Siekmann L, Klingmuller D, Characterization of the dehydroepiandrosterone (DHEA) metabolism via oxysterol 7 alpha-hyroxylase and 17-ketosteroid reductase activity in human brain. J Neurochem 83: 713–726.

    Article  PubMed  CAS  Google Scholar 

  33. Weill-Engerer S, David JP, Sazdovitch V, Liere P, Schumacher M, Delacourte A, Baulieu EE, Akwa Y, In vitro metabolism of dehydroepiandrosterone (DHEA) to 7 alpha-hyroxy- DHEA and Delta 5 androstene 3 beta, 17 beta-diol in specific regions of the aging brain from Alzheimer's and non-demented patients. Brain Res 969: 117–125, 2003

    Article  PubMed  CAS  Google Scholar 

  34. Mamczka M, Jung Y, Park BS, Partovi D, Reddy PH, Time-course of mitochondrial gene expressions in mice brains: implications for mitochondrial dysfunction, oxidative damage, and cytochrome c in aging. J Neurochem 92: 494–504, 2005

    Article  Google Scholar 

  35. Formoso G, Chen H, Kim JA, Montagnani M, Consoli A, Quon MJ, DHEA mimics acute actions of insulin to stimulate production of both NO and ET-1 via distinct PI 3-kinase and MAP-kinase-dependent pathways in vascular endothelium. Mol Endocrinol., 20: 1153–1163, 2006

    Google Scholar 

  36. Wang MJ, Huang HM, Chen HL, Kuo JS, Jeng KC, Dehydroepiandrosterone inhibits lipopolysaccharide-induced nitric oxide production in BV-2 microglia. J Neurochem 77:830–838, 2001

    Article  PubMed  CAS  Google Scholar 

  37. Kadenbach B, Intrinsic and extrinsic uncoupling of oxidative phosphorylation. Biochim Biophys Acta 1604: 77–94, 2003

    Article  PubMed  CAS  Google Scholar 

  38. Brunori M, Giuffre A, Forte E, Mastronicola D, Barone MC, Sarti P, Control of cytochrome c oxidase activity by nitric oxide. Biochim Biophys Acta 1655: 365–371, 2005

    Google Scholar 

  39. Aragno M, Cutrin JC, Mastrocola R, Perrelli MG, Restivo F, Poli G, Danni O, Boccuzzi G. Oxidative stress and kidney dysfunction due to ischemia/reperfusion in rat: attenuation by dehydroepiandrosterone. Kidney Int 64:836–843, 2003

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India

    Minal A. Patel & Surendra S. Katyare

Authors
  1. Minal A. Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Surendra S. Katyare
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Patel, M.A., Katyare, S.S. Treatment with dehydroepiandrosterone (DHEA) stimulates oxidative energy metabolism in the liver mitochondria from developing rats. Mol Cell Biochem 293, 193–201 (2006). https://doi.org/10.1007/s11010-006-9242-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-006-9242-3

Keywords

Search

Navigation