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Prior human polyomavirus and papillomavirus infection and incident lung cancer: a nested case–control study

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Abstract

Purpose

To test whether infection with select human polyomaviruses (HPyV) and human papillomaviruses (HPV) is associated with incident lung cancer.

Methods

We performed a nested case–control study, testing serum from the carotene and retinol efficacy trial, conducted 1985–2005, for antibodies to Merkel cell (MCV), KI (KIV), and WU (WUV) HPyVs as well as to six high-risk and two low-risk HPV types. Incident lung cancer cases (n = 200) were frequency-matched with controls (n = 200) on age, enrollment and blood draw dates, intervention arm assignment, and the number of serum freeze/thaw cycles. Sera were tested using multiplex liquid bead microarray antibody assays. We used logistic regression to assess the association between HPyV and HPV antibodies and lung cancer.

Results

There was no evidence of a positive association between levels of MCV, KIV, or WUV antibodies and incident lung cancer (p corrected >0.10 for all trend tests; odds ratio (OR) range 0.72–1.09, p corrected >0.10 for all). There was also no evidence for a positive association between HPV 16 or 18 infection and incident lung cancer (p corrected ≥0.10 for all trend tests; OR range 0.25–2.54, p > 0.05 for all OR > 1), but the number of persons with serologic evidence of these infections was small.

Conclusions

Prior infection with any of several types of HPyV or HPV was not associated with subsequent diagnosis of lung cancer. Infection with these viruses likely does not influence a person’s risk of lung cancer in Western smoking populations.

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Acknowledgments

We extend our gratitude to the study participants and CARET personnel who made this study possible. We also thank and acknowledge the contributions of Greg Wipf for assistance in the laboratory, and Margaret M. Madeleine, Lisa Johnson, and Alexa Resler for their consultation regarding study design and analytical methods. This work was supported by the Fogarty International Clinical Research Scholars and Fellows Program at Vanderbilt University [R24 TW007988 trainee support to D.V. Colombara]; the National Cancer Institute [R25 CA094880 trainee support to D.V. Colombara]; and the National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR000423 pilot award to L.E. Manhart and D.V. Colombara].

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Authors and Affiliations

  1. Institute for Health Metrics and Evaluation, University of Washington, 2301 Fifth Ave., Suite 600, Seattle, WA, 98121, USA

    Danny V. Colombara

  2. Department of Epidemiology, School of Public Health, University of Washington, 1959 NE Pacific Street, Health Sciences Building F-250, Box 357236, Seattle, WA, 98195-7236, USA

    Danny V. Colombara, Lisa E. Manhart, Stephen E. Hawes & Noel S. Weiss

  3. Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., P.O. Box 19024, Seattle, WA, 98109-1024, USA

    Danny V. Colombara, Joseph J. Carter, Noel S. Weiss, Matt J. Barnett, Gary E. Goodman & Denise A. Galloway

  4. Department of Biostatistics, School of Public Health, University of Washington, F-600, Health Sciences Building, Box 357232, Seattle, WA, 98195-7232, USA

    James P. Hughes

  5. Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, 135 Dauer Drive, 2101 McGavran-Greenberg Hall, CB #7435, Chapel Hill, NC, 27599-7435, USA

    Jennifer S. Smith

  6. Department of Cancer Epidemiology, Cancer Institute, Chinese Academy of Medical Sciences, 17 South Pan Jia Yuan Lane, Chaoyang Dist., P. O. Box 2258, Beijing, 100021, People’s Republic of China

    You-Lin Qiao

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  1. Danny V. Colombara
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  2. Lisa E. Manhart
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  3. Joseph J. Carter
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  4. Stephen E. Hawes
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  5. Noel S. Weiss
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  6. James P. Hughes
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  8. Gary E. Goodman
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  9. Jennifer S. Smith
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  11. Denise A. Galloway
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Corresponding author

Correspondence to Danny V. Colombara.

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Conflict of interest

LE Manhart has received test kits and reagents from Hologic/Gen-Probe over the past 5 years. JS Smith has received unrestricted educational grants, consultancy, and research grants from GlaxoSmithKline and Merck Corporation over the past 5 years. Y-L Qiao has received unrestricted educational grants, consultancy, and research grants from Qiagen, GlaxoSmithKline, and Merck Corporation over the past 5 years. All other authors report no potential conflicts.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Colombara, D.V., Manhart, L.E., Carter, J.J. et al. Prior human polyomavirus and papillomavirus infection and incident lung cancer: a nested case–control study. Cancer Causes Control 26, 1835–1844 (2015). https://doi.org/10.1007/s10552-015-0676-3

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  • DOI: https://doi.org/10.1007/s10552-015-0676-3

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