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Measurement of urinary Nτ-methylhistamine excretion: Correlation of a newly developed radioimmunoassay (RIA) with gas chromatography mass spectrometry (GCMS)

  • Histamine and Clinical Studies
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Abstract

A newly developed radioimmunoassay (RIA, Y) for the determination of urinary Nτ-methylhistamine concentrations was correlated with gas chromatography mass spectrometry (GCMS, X). In 34 urine samples, with histamine and Nτ-methylhistamine levels within our reference values, the correlation was: Y=1.47X−0.245 μmol/l (r=0.92;p-slope ≤0.0001). In 14 pathological urine samples, derived from patients with mastocytosis and having upper reference values, the correlation was: Y=1.75X−1.02 μmol/l (r=0.93;p-slope ≤0.001). In spite of the greater specificity of the monoclonal antibody for Nτ-methylhistamine compared with that of histamine, relatively high urinary histamine concentrations gave a false positive influence on the RIA results, which was 100% when the histamine/Nτ-methylhistamine ratio was about 19. Clear cases of mastocytosis can be diagnosed, using the RIA-kit, but for a more precise Nτ-methylhistamine value GCMS analyses will remain necessary.

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Authors and Affiliations

  1. ABL Analytical Biochemical Laboratory, P.O. Box 232, 9400 AE, Assen, The Netherlands

    E. Oosting

  2. Stiehting Diagnostisch Centrum Eindhoven, Eindhoven, The Netherlands

    J. J. Keyzer

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  1. E. Oosting
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  2. J. J. Keyzer
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Oosting, E., Keyzer, J.J. Measurement of urinary Nτ-methylhistamine excretion: Correlation of a newly developed radioimmunoassay (RIA) with gas chromatography mass spectrometry (GCMS). Agents and Actions 33, 215–217 (1991). https://doi.org/10.1007/BF01993171

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