Regular ArticleExpression of Adhesion Molecules in Cultured Human Pulmonary Microvascular Endothelial Cells
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Molecular mechanisms underlying the heterogeneous barrier responses of two primary endothelial cell types to sphingosine-1-phosphate
2022, European Journal of Cell BiologyCitation Excerpt :While this interest in EC heterogeneity included ECs from many organ systems, the ability to perform controlled studies of lung microvascular ECs was of particular importance due to the clinical burden of lung vascular dysfunction, including ARDS. Early reports of successful HPMEC isolation and culture came in the mid-1990s (Shen et al., 1995). Since then, HPMECs have been made commercially available for wide-spread experimentation.
The Balance Between Metalloproteinases and TIMPs: Critical Regulator of Microvascular Endothelial Cell Function in Health and Disease
2017, Progress in Molecular Biology and Translational ScienceCitation Excerpt :Under basal conditions, platelet endothelial cell adhesion molecule (PECAM)1 and ICAM1 are expressed on the MVEC surface.80–82 Under conditions of inflammation (e.g., TNFα stimulation), PECAM1 expression decreases while ICAM1 is significantly upregulated.83,84 Chemotactic stimuli released from the site of injury/infection, such as KC in mice or IL8 in humans, actively recruit PMN to the sites of injury/infection where they adhere to the endothelium in order to migrate into the surrounding tissue.47,80–82
The CD157-integrin partnership controls transendothelial migration and adhesion of human monocytes
2011, Journal of Biological ChemistryCitation Excerpt :Human monocytic THP-1 cell line (ATCC, Manassas, VA) was maintained in RPMI 1640 medium supplemented with 5% FCS. The human pulmonary microvascular endothelial cells (HPMEC) were generated as described (22). Cells (2 × 105/sample) were incubated for 30 min at 4 °C with 5 μg/ml of appropriate primary mAbs, washed, and incubated for 30 min at 4 °C with F(ab′)2 RαMIg-FITC.
Alphastatin, a 24-amino acid fragment of human fibrinogen, is a potent new inhibitor of activated endothelial cells in vitro and in vivo
2004, BloodCitation Excerpt :Twenty-four–well plates were coated with 30 μL/well of growth factor–reduced (GF-reduced) Matrigel (Becton Dickinson Labware, Bedford, MA). Endothelial cells on this matrix migrated and formed tubules within 6 hours of plating.17 Cells (HuDMECs and SVEC4-10) were seeded at 4 × 104 cells/mL and incubated for 6 hours in 50 μL DMEM + 1% FCS alone (control) or medium ± 10, 100, 1000 nM alphastatin and with or without 10 ng/mL bFGF or VEGF.
Comparative Study of Adhesion Molecule Expression in Cultured Human Macro- and Microvascular Endothelial Cells
2002, Experimental and Molecular Pathology