A Novel Gene, CRR9, Which Was Up-Regulated in CDDP-Resistant Ovarian Tumor Cell Line, Was Associated with Apoptosis

doi:10.1006/bbrc.2001.4250

Biochemical and Biophysical Research Communications

Volume 280, Issue 4, 2 February 2001, Pages 1148-1154

https://doi.org/10.1006/bbrc.2001.4250 Get rights and content

Abstract

In the screening for cisplatin (CDDP)-resistance related genes by a mRNA differential display method, we detected some increased bands in CDDP resistant ovarian tumor cell line 2008/C13*5.25. One of them, named DD9, was a positive fragment on Northern blot analysis. We cloned it as a full length cDNA by 5′RACE and found a novel gene, CRR9 (Cisplatin Resistance Related gene 9). The CRR9 gene was transcribed into a 2.0 kb mRNA, encoding 512 amino acids. The putative protein had transmembrane-like domains and well conserved on C terminus with human CLPTM1 and the homologs found in Drosophila and C. elegans. Transfection assay showed that the CDDP-sensitive strain 2008 with CRR9 was more sensitive to CDDP, indicating that CRR9 was not associated with the CDDP-resistance, but the CDDP-induced apoptosis.

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Citation Excerpt :
As a telomerase regulatory gene, TERT encodes the catalytic subunit of telomerase and plays vital roles in maintaining telomerase expression and facilitating telomeric repeats elongation (Robles-Espinoza et al., 2015). CLPTM1L encodes the cleft lip and palate-associated transmembrane 1 like protein, which is associated with cisplatin-induced apoptosis (Yamamoto et al., 2001). Given the above findings that multiple metals exposure and genetic variations in the TERT-CLPTM1L cluster were both important determinants on telomere length, a critical question of interest is to integrate genome and exposome information in order to understand their combined effects.
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^☆: Abbreviations used: ABC, ATP binding cassette; CDDP, cisplatin; GFP, green fluorescent protein; HSP, heat shock protein; MDR, multi drug resistance; MLH, mutL homolog; MSH, mutS homolog; MRP, multi drug resistant protein; RACE, rapid amplification of cDNA ends.

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Regular ArticleA Novel Gene, CRR9, Which Was Up-Regulated in CDDP-Resistant Ovarian Tumor Cell Line, Was Associated with Apoptosis☆

Abstract

Exp. Cell Res.

Genomics

A human canalicular multispecific organic anion transporter (cMOAT) gene is overexpressed in cisplatin-resistant human cancer cell lines with decreased drug accumulation

Cancer Res.

Expression of gamma-glutamylcysteine synthetase (gamma-GCS) and multidrug resistance-associated protein (MRP), but not human canalicular multispecific organic anion transporter (cMOAT), genes correlates with exposure of human lung cancers to platinum drugs

Br. J. Cancer

Human MutSalpha recognizes damaged DNA base pairs containing O6-methylguanine, O4-methylthymine, or the cisplatin-d(GpG) adduct

Proc. Natl. Acad. Sci. USA

Regular Article
A Novel Gene, CRR9, Which Was Up-Regulated in CDDP-Resistant Ovarian Tumor Cell Line, Was Associated with Apoptosis☆