Table 2– Primer sequences and amplicon sizes for human tissues^#

Gene	Accession	Sequences (5′ → 3′)	Length bp	Amplicon bp
DKK1	NM012242	For: CGCCGAAAACGCTGCAT	17	109
DKK1	NM012242	Rev: TTTCCTCAATTTCTCCTCGGAA	22	109
DKK2	NM014421	For: TCAGGCCGCCAATCGA	16	85
DKK2	NM014421	Rev: GTAGGCCTGCCCCAGGTT	18	85
DKK3	NM015881	For: GCTTCTGGACCTCATCACCTG	21	119
DKK3	NM015881	Rev: TCGGCTTGCACACATACACC	20	119
DKK4	NM014420	For: GAAGGGCTCACAGTGCCTGT	20	131
DKK4	NM014420	Rev: AGCACATGGCATCTCGCTG	19	131
KRM1	NM001039570	For: TGGAAGCCACAGAGTTGAAGG	21	146
KRM1	NM001039570	Rev: GACAATCCCTAAGGTCCCCTG	21	146
KRM2	NM172229	For: CTGGCGCTACTGCGACATC	19	62
KRM2	NM172229	Rev: AGTCCACAAAGCATCCCAGGTA	22	62
HPRT1	NM000194	For: AAGGACCCCACGAAGTGTTG	20	157
HPRT1	NM000194	Rev: GGCTTTGTATTTTGCTTTTCCA	22	157

DKK: Dickkopf; KRM: Kremen; HPRT1: hypoxanthine guanine phosphoribosyl transferase 1; for: forward; rev: reverse. ^#: All primer sets worked under identical real-time PCR cycling conditions with similar efficiencies to obtain simultaneous amplification in the same run. Sequences were taken from GeneBank, all accession numbers are denoted.