Table 2– Isolation frequencies for the microorganisms in metalworking fluid (MWF), water and additive samples^#

Sample type	Positive samples	Sterile samples	Mycobacterium immunogenum	Mycobacterium llatzerense	Aspergillus fumigatus	Fusarium solani	Other fungi^¶	Bacillus spp.	Other bacteria⁺
New MWF	0	4	0	0	0	0	0	0	0
Additive	0	1	0	0	0	0	0	0	0
Used MWF	74	9	33	0	4	3	3	35	9
Industrial water	2	0	0	0	0	0	0	0	2
Osmosed water	5	0	0	1	0	0	1	1	5
Ultrafiltered water	2	0	0	0	0	0	0	0	2

Data are presented as n. ^#: n = 97; ^¶: Aspergillus flavus (n = 1), Curvularia lunata (n = 1), Acremonium sp. (n = 1) and Penicillium sp. (n = 1); ⁺: nonmycobacteria and nonactinomycetes.