Table 1– Potency values of aclidinium as antagonist of carbachol- and cigarette smoke-induced augmentation of the expression of mucin MUC5AC mRNA and protein expression in human isolated bronchial tissue and human airway epithelial cells cultured in air–liquid interface system
Potency values as inhibitor of
MUC5AC mRNA expressionMUC5AC protein expression
Carbachol in human isolated bronchial tissues
 Aclidinium9.17±0.148.81±0.16
 Atropine8.61±0.088.78±0.09
Carbachol in human cultured airway epithelial cells
 Aclidinium9.13±0.128.95±0.09
 Atropine8.69±0.058.60±0.06
Cigarette smoke in human cultured airway epithelial cells
 Aclidinium8.80±0.128.67±0.11
 Atropine8.29±0.148.24±0.13

  • Data are presented as mean±sem of 3–5 independent experiments. The corresponding values for atropine are also given for comparison. Potency values are given as the -log IC50 values calculated from the concentration-dependent inhibition of the carbachol 100 μM and cigarette smoke 10%-induced overexpression of MUC5AC mRNA and protein obtained in the presence of aclidinium (0.1, 1, 10 and 100 nM) and atropine (1, 10, 100 and 1000 nM) under the experimental conditions described in the methods section.