16HBE14o- | BEAS-2B | NCI-H292 | A549 | PBECs | |
Resistance (400 Hz) at 72 h | 12000–20000 | 1400–1600 | 1000–1200 | 1200–1500 | 2000–5000 |
Time to confluence# h | 30–50 | 2.5–20 | 30–40 | 2.5–15 | 2–10 |
ZO-1 at 72 h | |||||
Expression | High | Low | Moderate | Low | Moderate |
Localisation at junctions | Yes | Fragmented | No | No | Yes |
F-actin organisation | Cortical | Stress fibres | Cortical | Stress fibres | Stress fibres |
PBEC: primary bronchial epithelial cell; ZO: zona occludens (tight junction protein); F-actin: filamentous actin. #: time taken to cover electrode following seeding at 7.5×104 cells·well−1.