geNorm | BestKeeper | NormFinder | |||
Gene | Mean M¶ | Gene | r+ | Gene | Stability value |
PPIA/RPLP0 | 0.416 | PPIA | 0.988 | PPIA | 0.111 |
PGK1 | 0.984 | PGK1 | 0.129 | ||
GNB2L1 | 0.449 | B2M | 0.956 | RPLP0 | 0.169 |
PGK1 | 0.488 | GUSB | 0.953 | GNB2L1 | 0.209 |
GUSB | 0.528 | GNB2L1 | 0.953 | GUSB | 0.246 |
B2M | 0.564 | RPLP0 | 0.952 | B2M | 0.258 |
RNR1 | 0.615 | RNR1 | 0.925 | RNR1 | 0.323 |
GAPDH | 0.684 | ACTB | 0.915 | ACTB | 0.396 |
ACTB | 0.716 | GAPDH | 0.913 | GAPDH | 0.405 |
TFRC | 0.903 | TFRC | 0.847 | TFRC | 0.594 |
M: gene expression stability. PPIA: cyclophilin A (or peptidylprolyl isomerase A) gene; RPLP0: acidic ribosomal protein gene; GNB2L1: guanine nucleotide-binding protein, β-peptide 2-like 1, gene; PGK1: phosphoglycerokinase gene; GUSB: β-glucuronidase gene; B2M: β2-microglobulin gene; RNR1: 18S ribosomal RNA gene; GAPDH: glyceraldehyde-3-phosphate dehydrogenase gene; ACTB: β-actin gene; TFRC: transferrin receptor gene. #: genes listed from most to least stable for all three methods; ¶: stability value; +: all 10 candidate HKGs were highly correlated and were combined to calculate the BestKeeper index, which was then used to determine the correlation between each HKG and the index.