Primer sequences and amplicon sizes used for the analysis of human lung tissues and pulmonary arterial smooth muscle cells
| Gene | Accession No. | Forward primer | Reverse primer | Amplicon size bp | ||
| Sequence | Length bp | Sequence | Length bp | |||
| Has1 | NM001523 | gcgatactgggtagccttca | 20 | ggttgtaccaggcctcaaga | 20 | 131 |
| Has2 | NM005328.1 | acagacaggctgaggacgac | 20 | ctgtgattccaaggaggag | 20 | 126 |
| Has3 | NM005329.2 | gtcatgtacacggccttcaa | 20 | cctacttggggatcctcctc | 20 | 125 |
| Hyal1 | NM007312 | gtgctgccctatgtccagat | 20 | attttcccagctcacccaga | 20 | 132 |
| Hyal2 | NM033158 | tctaccattggcgagagtg | 19 | gcagccgtgtcaggtaat | 19 | 119 |
| Hyal3 | NM003549 | gatctgggaggttcctgtcc | 20 | agagctggagaggctcaggt | 20 | 110 |
| Hyal4 | NM012269 | tgaggatctccaccatgaca | 20 | ggcagcactttctcctatgg | 20 | 134 |
| Cd44 | NM000610.3 | cccagatggagaaagctctg | 20 | gttgtttgctgcacagatgg | 20 | 113 |
| Rhamm | NM012484.1 | gttgtgcaccatctccaggt | 20 | agctgaagcaggcaaggtag | 20 | 152 |
| Hmbs | NM000190.3 | gcacccacacacagcctac | 19 | gtacccacgcgaatcactct | 20 | 108 |
All primer sets were designed to work under identical real-time PCR cycling conditions and simultaneous amplifications were obtained in the same run. Sequences were according to GeneBank reference sequences. Primer sequences are given in 5′–3′ orientation. Has: hyaluronan synthase; Hyal: hyaluronoglucosaminidase; Rhamm: receptor for hyaluronan-mediated motility; Hmbs: hydroxymethylbilane synthase.