Sequences of forward and reverse primers used for semi-quantitative and quantitative real-time RT-PCR
| Gene name | Forward primer 5′-3′ | Reverse primer 5′-3′ | GenBank accession number |
| mouse acta2 | GCTGGTGATGATGCTCCCA | GCCCATTCCAACCATTACTCC | NM_007392 |
| mouse pbgd | GGTACAAGGCTTTCAGCATCGC | ATGTCCGGTAACGGCGGC | NM_013551 |
| mouse pgk1 | TGGGACTGTCATCCTGCTGG | GAGGCTCGGAAAGCATCAAT | NM_008828 |
| mouse shrm | ACTGTCCAGGCTGTTCCTCAA | CAGCCAGCCGGTTTGGT | NM_027917 |
| human acta2 | CGAGATCTCACTGACTACCTCATGA | AGAGCTACATAACACAGTTTCTCCTTGA | NM_001613 |
| human shrm1 | AGGCGAAGTCTTGGGTTCCT | TGAGGCAAGAACCTGGAAAGTT | NM_133456 |
| human shrm3 | TGGATCTAGGTCACTCAGTTGTTCA | AGGAACGGAGGAATCACCAA | NM_020859 |
| human hprt | AAGGACCCCACGAAGTGTTG | GGGTTTGTATTTTGCTTTTCC | NM_000194 |
acta: smooth muscle alpha-actin; pbgd: porphobilinogen deaminase; pgk: phosphoglycerate kinase; shrm: Shroom; hprt: hypoxanthine–guanine phosphoribosyltransferase.