Table 1—

Antibodies and processing used in immunohistochemical analyses

AntibodyPre-treatmentSpeciesDilutionCloneOrigin
Collagen ICitrate/pepsinGoat1:2000PolyclonalUS Biological, Swampscott, MA, USA
Collagen IIITrypsinMouse1:2500III-53Oncogene and Calbiochem, Darmstadt, Germany
FibronectinTrypsinRabbit1:4000PolyclonalDako, Glostrup, Denmark
VersicanNoneMouse1:20002-b-1Seikagaku Co., Tokyo, Japan
MMP-1CitrateMouse1:150041-1E5Oncogene and Calbiochem
MMP-2CitrateMouse1:3000A-Gel VC2Labvision, Fremont, CA, USA
MMP-9CitrateMouse1:40626–644Oncogene and Calbiochem
MMP-12CitrateMouse1:104D2R&D Systems, Minneapolis, MN, USA
TIMP-1NoneMouse1:252A5Labvision
TIMP-2TrypsinMouse1:1003A4Labvision
  • MMP: matrix metalloproteinase; TIMP: tissue inhibitor of metalloproteinase.