Table 1—

Oligonucleotides used in conventional and real-time quantitative PCR

NameSequence
Conventional PCR
 α-ENaC forward primerGCCTGTCTGCGTCTAAAGC
 α-ENaC reverse primerAGTGGACTGTGGAGGGCTA
 β-Actin forward primerCATGAAGATCCTCACCGAGC
 β-Actin reverse primerGTGGACATCCGCAAAGACCT
Real-time quantitative PCR</EMPH>
 α-ENaC forward primerGAGATTCCTGTCGCTTCC
 α-ENaC reverse primerCTGTGGAGGGCTAGAGTC
 GAPDH forward primerTTGATTTTGGAGGGATCTCG
 GAPDH reverse primerCAATGACCCCTTCATTGACC

  • GAPDH: glyceraldehyde-3-phosphate dehydrogenase.