Table 5—

Inhibition of anti-heparan sulphate antibody binding by pre-incubation with growth factors

PatientPre-incubation with IGF-1Pre-incubation with HGF
HS4C3AO4B08HS4C3AO4B08
Non-COPD
 1+/−++/−+
 2+/−++
 3+/−++
 4+/−++/−+
 5+/−++
COPD
 6+/−++/−+
 7+/−++
 8+/−++/−+
 9+/−++
 10+/−++
 11+++
 12+++
 13+++
 14+++
  • IGF: insulin-like growth factor; HGF: hepatocyte growth factor; COPD: chronic obstructive pulmonary disease. Cryosections were pre-incubated with growth factors IGF-1 or HGF, followed by incubation with HS4C3 or AO4B08. Bound antibodies were visualised. An overall inhibition was seen for HS4C3 after pre-incubation with HGF. After pre-incubation with IGF-1, HS4C3 was slightly inhibited but AO4B08 was not. +: comparable intensity to non-pre-incubated cryosections; +/−: reduced intensity compared with non-pre-incubated cryosections; −: completely absent staining.