Table 1—

Categories for computerised point counting of haematoxylin and eosin stained cross-sections of the diaphragm

FeaturesDefinition
Normal
 Peripheral nucleusNucleus situated within 2 µm of sarcolemma
 Normal cytoplasmEvenly textured, eosinophilic cytoplasm
 Normal size and shapeHomogenous size, polygonal shape
Abnormal
 Internal nucleusMinimum 2 µm of cytoplasm between nucleus and sarcolemma
 Abnormal cytoplasmPale staining or basophilic cytoplasm; granular, whorled, vacuolated or disrupted texture; loss of sarcolemma integrity; lipofuscin accumulation; rounded, deeply eosinophilic fibre
 Abnormal size or shapeSmall fibre (<1/3 diameter of three largest fibres in the field), angulated fibre with concave edges, lobulated fibre