Table 2—

Bronchoalveolar lavage(BAL) analysis and lung function parameters

Samples used for RT-PCR analysesSamples used for CBA analysesControls
HLA-DRB1*0301+DRB1*0301-DRB1*0301+DRB1*0301-For RT-PCR and CBA
Subjects n1213142011
Radiograph stage I/II/III n6/6/02/9/26/8/04/13/3ND
BAL analysis
 Recovery %70 (63–89)68 (51–83)73 (54–89)68 (16–85)74 (63–88)
 Viability %93 (80–99)94 (85–100)95 (80–100)96 (85–100)92 (87–98)
 Cell concentration ×106·L−1198 (104–481)270 (89–746)161 (93–319)245 (133–746)72 (47–165)***
Differential cell counts
 Macrophages %67 (45–94)55 (38–87)65 (45–95)56 (38–87)89 (80–95)**
 Lymphocytes %32 (5–52)38 (11–60)34 (4–50)39 (11–60)9 (6–20)**
 Neutrophils %1 (0–4)1 (0–8)1 (0.2–4.2)0.9 (0–34)1 (0–2)
 Eosinophils %0 (0–3)0 (0–3)0.4 (0.2–3.2)0.3 (0–1.2)0 (0–1)
 CD4/CD8 ratio5 (2–19)6 (1–21)7 (2–31)5 (1–21)ND
Pulmonary function tests % of reference value
 VC89.5 (80–110)85.5 (64–108)91 (80–110)*87 (65–108)ND
 FEV188.5 (80–105)83.5 (68–109)89 (80–105)80.5 (47–109)ND
DL,CO91 (85–119)*73.5 (56–114)87 (74–119)78 (56–114)ND
  • Data are presented as median (minimum–maximum), unless otherwise stated. RT: real time; CBA: cytometric bead array; ND: not done; VC: vital capacity; FEV1: forced expiratory volume in one second; DL,CO: diffusing capacity of the lung for carbon monoxide. *: p<0.05 between patient subgroups; **: p<0.01 versus each patient subgroup; ***: p<0.001 versus each patient subgroup.