Antibody | Marker | Dilution | Clone | Source |
CD3 | CD3, T-lymphocytes | 1:2000 | UCHT 1 | Dako, Glostrup, Denmark |
CD4 | CD4, T-helper cells | 1:20 | MT310 | Dako, Glostrup, Denmark |
CD8 | CD8, cytotoxic T-cells | 1:40 | C8/144B | Dako, Glostrup, Denmark |
CD25 | IL-2 receptor | 1:50 | ACT-1 | Dako, Glostrup, Denmark |
CD14 | CD14, macrophages | 1:8 | TUK4 | Dako, Glostrup, Denmark |
AA1 | Tryptase, mast cells | 1:200 | AA1 | Dako, Glostrup, Denmark |
NE | Elastase, neutrophils | 1:300 | NP57 | Dako, Glostrup, Denmark |
IL-8 | IL-8 | 1:100 | NAP II | Boehringer Ingelheim, Heidelberg, Germany |
Gro-α | Gro-α | 1:350 | 20326.1 | R&D, Abingdon, UK |
TNF-α | TNF-α | 1:85 | 28401.111 | R&D, Abingdon, UK |
IL-18 | IL-18 | 1:60 | 125-2H | R&D, Abingdon, UK |
CD62E | E-selectin, microvasculature | 1:10 | 1.2B6 | Dako, Glostrup, Denmark |
CD62P | P-selectin, microvasculature | 1:20 | AK-6 | Serotec, Oxford, UK |
CD54 | ICAM-1, microvasculature | 1:800 | 6.5B5 | Dako, Glostrup, Denmark |
VCAM-1 | VCAM-1, microvasculature | 1:10 | 1.4C3 | Dako, Glostrup, Denmark |
EN4 | Endothelium, microvasculature | 1:40 | EN4 | Monosan, Uden, The Netherlands |
p-p38 | p38 | 1:20 | D-8 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
p-JNK | c-jun N-terminal kinase | 1:20 | G-7 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
p-ERK | Extracellular signal-regulated protein kinase | 1:30 | E-4 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
p65 | Nuclear factor-κB | 1:25 | G96-347 | Becton Dickinson–Pharmingen, San Diego, CA, USA |
p-C-Jun | Activator protein-1 | 1:35 | KM-1 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
C-Fos | Activator protein-1 | 1:10 | D-1 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
IL: interleukin; Gro: growth-related oncogene; TNF: tumour necrosis factor; ICAM: intercellular adhesion molecule; VCAM: vascular cell adhesion molecule. #: against inflammatory cells, cytokines, adhesion molecules, mitogen-activated protein kinases and transcription factors.