Table. 1—

Monoclonal antibodies# used in the immunohistochemical analysis

AntibodyMarkerDilutionCloneSource
CD3CD3, T-lymphocytes1:2000UCHT 1Dako, Glostrup, Denmark
CD4CD4, T-helper cells1:20MT310Dako, Glostrup, Denmark
CD8CD8, cytotoxic T-cells1:40C8/144BDako, Glostrup, Denmark
CD25IL-2 receptor1:50ACT-1Dako, Glostrup, Denmark
CD14CD14, macrophages1:8TUK4Dako, Glostrup, Denmark
AA1Tryptase, mast cells1:200AA1Dako, Glostrup, Denmark
NEElastase, neutrophils1:300NP57Dako, Glostrup, Denmark
IL-8IL-81:100NAP IIBoehringer Ingelheim, Heidelberg, Germany
Gro-αGro-α1:35020326.1R&D, Abingdon, UK
TNF-αTNF-α1:8528401.111R&D, Abingdon, UK
IL-18IL-181:60125-2HR&D, Abingdon, UK
CD62EE-selectin, microvasculature1:101.2B6Dako, Glostrup, Denmark
CD62PP-selectin, microvasculature1:20AK-6Serotec, Oxford, UK
CD54ICAM-1, microvasculature1:8006.5B5Dako, Glostrup, Denmark
VCAM-1VCAM-1, microvasculature1:101.4C3Dako, Glostrup, Denmark
EN4Endothelium, microvasculature1:40EN4Monosan, Uden, The Netherlands
p-p38p381:20D-8Santa Cruz Biotechnology, Santa Cruz, CA, USA
p-JNKc-jun N-terminal kinase1:20G-7Santa Cruz Biotechnology, Santa Cruz, CA, USA
p-ERKExtracellular signal-regulated protein kinase1:30E-4Santa Cruz Biotechnology, Santa Cruz, CA, USA
p65Nuclear factor-κB1:25G96-347Becton Dickinson–Pharmingen, San Diego, CA, USA
p-C-JunActivator protein-11:35KM-1Santa Cruz Biotechnology, Santa Cruz, CA, USA
C-FosActivator protein-11:10D-1Santa Cruz Biotechnology, Santa Cruz, CA, USA
  • IL: interleukin; Gro: growth-related oncogene; TNF: tumour necrosis factor; ICAM: intercellular adhesion molecule; VCAM: vascular cell adhesion molecule. #: against inflammatory cells, cytokines, adhesion molecules, mitogen-activated protein kinases and transcription factors.